Primer . | Nucleotide sequence (5′→3′) . | Direction +/– . | Acc. no X62405 nucleotide position (sand goby rhodopsin numbering) . |
---|---|---|---|
SG1* | TGA ATT CAC CGC CAA CAA AC | + | 19–38 |
SG2* | ATG ATC CAG GAG AAA GCC AC | – | 514–533 |
SG3† | CAC CGC CAA CAA ACC GCA AC | + | 25–44 |
SG4† | AGA ATT TAG TGG TTT CCC AG | – | 1147–1166 |
SG5‡ | ATG AAC GGC ACG GAG GGA CC | + | 46–65 |
SG6‡ | ATG AAC GGT ACG GAG GGA CC | + | 46–65 |
SG7C‡ | AGA GGC GGC ACA GCA CAG GT | – | 541–560 |
SG8§ | CTG CAA ACC CAT CAG CAA CT | + | 462–481 |
SG9§ | GTG AGT GTC GGT CTT GTT TTA | – | 1102–1122 |
SG10** | TCC ACT CCA CAT GAG CAC TG | – | 595–615 |
Primer . | Nucleotide sequence (5′→3′) . | Direction +/– . | Acc. no X62405 nucleotide position (sand goby rhodopsin numbering) . |
---|---|---|---|
SG1* | TGA ATT CAC CGC CAA CAA AC | + | 19–38 |
SG2* | ATG ATC CAG GAG AAA GCC AC | – | 514–533 |
SG3† | CAC CGC CAA CAA ACC GCA AC | + | 25–44 |
SG4† | AGA ATT TAG TGG TTT CCC AG | – | 1147–1166 |
SG5‡ | ATG AAC GGC ACG GAG GGA CC | + | 46–65 |
SG6‡ | ATG AAC GGT ACG GAG GGA CC | + | 46–65 |
SG7C‡ | AGA GGC GGC ACA GCA CAG GT | – | 541–560 |
SG8§ | CTG CAA ACC CAT CAG CAA CT | + | 462–481 |
SG9§ | GTG AGT GTC GGT CTT GTT TTA | – | 1102–1122 |
SG10** | TCC ACT CCA CAT GAG CAC TG | – | 595–615 |
Altogether, three different PCR primer pairs were used(†,‡,§)
Original nucleotide numbering system follows that of Archer et al., 1992. The open reading frame begins from base position 46 and ends at position 1102
Original PCR- and sequencing primer pair
Primer pair for most of the PCR and sequencing reactions, whole gene
Primer pair used for the Adriatic sand gobies for the PCR and sequencing reactions, first part
Primer pair used for the Adriatic sand gobies for the PCR and sequencing reactions, last part
Additional primer for the sequencing reactions