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Table 1.

Metabolite levels and enzyme activities in liver of rainbow trout sampled 6 h after intracerebroventricular injection into the third ventricle of 1 μl 100 g–1 body mass of Cortland saline alone (control) or containing d-glucose (400 μg μl–1) or l-(+)-lactate (400 μg μl–1)

ICV Treatment
ParameterControlGlucoseLactate
Metabolite levels    
   Glucose (μmol g–1 wet mass) 4.73±0.56a 2.73±0.29b 3.68±0.42a,b 
   Glycogen (μmol g–1 wet mass) 85.9±23.9a 13.6±4.75b 100.5±26.2a 
   Lactate (μmol g–1 wet mass) 3.07±0.21a,b 3.89±0.24b 2.91±0.20a 
   α-Amino acid (μmol g–1 wet mass) 31.2±1.18a 24.5±1.38b 22.2±1.10b 
   DHAP (nmol g–1 wet mass) 27.6±5.56a 56.9±9.20b 29.5±4.72a 
   GAP (nmol g–1 wet mass) 17.4±2.68 24.7±2.87 34.2±4.93 
   Glucose 6-phosphate (μmol g–1 wet mass) 1.18±0.14a 0.65±0.08b 2.20±0.36c 
Enzyme activities    
   GK (mi.u. mg–1 protein) 9.83±0.70a,b 9.52±0.98a 7.95±0.42b 
   Low Km HK (mi.u. mg–1protein) 0.37±0.03 0.35±0.04 0.29±0.04 
   LDH-O (i.u. mg–1 protein) 1.02±0.08a 0.94±0.05a 0.70±0.09b 
   LDH-R (i.u. mg–1 protein) 33.4±1.00a 27.9±1.96b 36.1±1.41a 
   PK (i.u. mg–1 protein) 0.75±0.04a 0.65±0.05a 0.39±0.02b 
   FBPase (i.u. mg–1 protein) 0.25±0.02 0.22±0.02 0.27±0.02 
   G6Pase (mi.u. mg–1 protein) 4.53±0.31a 3.19±0.32b 3.33±0.32b 
   GSase    
      Total activity (mi.u. mg–1 protein) 352±34.8a 400±36.8a 234±15.5b 
      % GSasea 13.9±1.59a 22.7±2.05b 26.7±3.09b 
   GPase    
      Total activity (mi.u. mg–1 protein) 63.2±6.97 124±18.4 109±14.2 
      % GPasea 48.4±7.13 49.7±2.37 55.7±5.81 
ICV Treatment
ParameterControlGlucoseLactate
Metabolite levels    
   Glucose (μmol g–1 wet mass) 4.73±0.56a 2.73±0.29b 3.68±0.42a,b 
   Glycogen (μmol g–1 wet mass) 85.9±23.9a 13.6±4.75b 100.5±26.2a 
   Lactate (μmol g–1 wet mass) 3.07±0.21a,b 3.89±0.24b 2.91±0.20a 
   α-Amino acid (μmol g–1 wet mass) 31.2±1.18a 24.5±1.38b 22.2±1.10b 
   DHAP (nmol g–1 wet mass) 27.6±5.56a 56.9±9.20b 29.5±4.72a 
   GAP (nmol g–1 wet mass) 17.4±2.68 24.7±2.87 34.2±4.93 
   Glucose 6-phosphate (μmol g–1 wet mass) 1.18±0.14a 0.65±0.08b 2.20±0.36c 
Enzyme activities    
   GK (mi.u. mg–1 protein) 9.83±0.70a,b 9.52±0.98a 7.95±0.42b 
   Low Km HK (mi.u. mg–1protein) 0.37±0.03 0.35±0.04 0.29±0.04 
   LDH-O (i.u. mg–1 protein) 1.02±0.08a 0.94±0.05a 0.70±0.09b 
   LDH-R (i.u. mg–1 protein) 33.4±1.00a 27.9±1.96b 36.1±1.41a 
   PK (i.u. mg–1 protein) 0.75±0.04a 0.65±0.05a 0.39±0.02b 
   FBPase (i.u. mg–1 protein) 0.25±0.02 0.22±0.02 0.27±0.02 
   G6Pase (mi.u. mg–1 protein) 4.53±0.31a 3.19±0.32b 3.33±0.32b 
   GSase    
      Total activity (mi.u. mg–1 protein) 352±34.8a 400±36.8a 234±15.5b 
      % GSasea 13.9±1.59a 22.7±2.05b 26.7±3.09b 
   GPase    
      Total activity (mi.u. mg–1 protein) 63.2±6.97 124±18.4 109±14.2 
      % GPasea 48.4±7.13 49.7±2.37 55.7±5.81 

ICV treatment, intracerebroventricular injection; DHAP, di-hydroxiacetone phosphate; GAP, glyceraldehyde 3-phosphate; GK, glucokinase; HK, hexokinase 1;LDH-O, lactate dehydrogenase oxidase; LDH-R, lactate dehydrogenase reductase;PK, pyruvate kinase; FBPase, fructose 1,6-bisphosphatase; G6Pase, glucose 6-phosphatase; GSase, glycogen synthase; GPase, glycogen phosphorylase

Each value is the mean ± s.e.m. of N=8 fish per group. When necessary (ratios) values were log transformed prior to statistical analysis. Different letters indicate significant differences among treated groups(one-way ANOVA, P<0.05)

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