The Company of Biologists

Table 1.

MWC and derived parameters for Carcinus maenas hemolymph with 0,10 and 50 mmol l^–1 L-lactate at 10°C

[L-lactate] (mmol l^–1)	pH	P₅₀ (mmHg)	P_m (mmHg)	n₅₀	n_max	logK_T	logSE	logK_R	logSE	logL	q^{^*}	ΔG (kJ mol^–1)
A
0	7.83	7.81	7.83	3.4	3.4	–1.9651	0.05169	0.1483	0.0879	4.6990	4.51	11.39
10	7.85	4.66	4.69	2.6	2.6	–1.3854	0.0839	0.0128	0.1010	3.1980	4.67	7.51
50	7.85	3.00	3.11	2.0	2.0	–1.4589	0.2894	0.0903	0.0455	1.8727	3.22	7.45
B
0	7.83	7.69	7.70	3.2	3.2	–1.9861	0.0505	0.2055	0.0775	4.3670	4	11.84
10	7.85	4.63	4.67	2.4	2.4	–1.4364	0.0700	0.0495	0.0817	2.8739	4	7.90
50	7.85	3.00	3.10	2.2	2.2	–1.2314	0.0604	0.0708	0.0347	2.2671	4	6.77

[L-lactate] (mmol l^–1)	pH	P₅₀ (mmHg)	P_m (mmHg)	n₅₀	n_max	logK_T	logSE	logK_R	logSE	logL	q^{^*}	ΔG (kJ mol^–1)
A
0	7.83	7.81	7.83	3.4	3.4	–1.9651	0.05169	0.1483	0.0879	4.6990	4.51	11.39
10	7.85	4.66	4.69	2.6	2.6	–1.3854	0.0839	0.0128	0.1010	3.1980	4.67	7.51
50	7.85	3.00	3.11	2.0	2.0	–1.4589	0.2894	0.0903	0.0455	1.8727	3.22	7.45
B
0	7.83	7.69	7.70	3.2	3.2	–1.9861	0.0505	0.2055	0.0775	4.3670	4	11.84
10	7.85	4.63	4.67	2.4	2.4	–1.4364	0.0700	0.0495	0.0817	2.8739	4	7.90
50	7.85	3.00	3.10	2.2	2.2	–1.2314	0.0604	0.0708	0.0347	2.2671	4	6.77

SE, standard errors (of K_T and K_Rvalues); for other abbreviations, see text

The MWC model was fitted to the data with q (the number of interacting O₂-binding sites) floating (A) or fixed at 4 (B)