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Table 1.

Metal accumulation in Drosophila

Cu limitation*
Basal
6 mmol l–1Cu
w1118Mvl97fw1118Mvl97fw1118Mvl97f
Cu 2.24±0.09 2.04±0.05 3.41±0.08 3.19±0.09 31.2±1.16 63.1±2.66§ 
Fe 32.2±1.43 23.5±1.84§ 35.5±1.76 25.3±1.39§ 33.9±1.54 37.6±2.71 
Mn 4.91±0.12 7.62±1.61 3.96±0.10 4.11±0.22 3.40±0.43 5.48±0.23 
Zn 13.4±0.43 14.1±0.56 14.6±0.29 14.4±0.53 8.89±0.57 13.1±1.41§ 
Cu limitation*
Basal
6 mmol l–1Cu
w1118Mvl97fw1118Mvl97fw1118Mvl97f
Cu 2.24±0.09 2.04±0.05 3.41±0.08 3.19±0.09 31.2±1.16 63.1±2.66§ 
Fe 32.2±1.43 23.5±1.84§ 35.5±1.76 25.3±1.39§ 33.9±1.54 37.6±2.71 
Mn 4.91±0.12 7.62±1.61 3.96±0.10 4.11±0.22 3.40±0.43 5.48±0.23 
Zn 13.4±0.43 14.1±0.56 14.6±0.29 14.4±0.53 8.89±0.57 13.1±1.41§ 

Values are mean ± s.e.m. of five replicates of 50 Drosophila. An independent samples t-test was used to determine significant differences. P<0.05, §P<0.01 compared to w1118control

*

Measurements were made on pupae raised on medium containing 100 μmol l–1 bathocuproinedisulfonic acid (BCS) to limit Cu availability and compared to pupae raised on basal medium

Response to excess copper was measured in 7-day-old adults after a 24 h exposure to medium containing excess Cu (6 mmol l–1). Cu, Fe,Mn and Zn levels were determined by ICP-AES and expressed as ng/fly

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