Primers used for qPCR and cloning of toadfish SLC26a6
| Primer . | Sequence (5′→3′) . | Product size (bp) . |
|---|---|---|
| Eel SLC26a6-F* | TGGTGCGGTTTGGATTTGTG | 544 |
| Eel SLC26a6-R* | ACCAGTTCCTGGTTGCTGTC | |
| SLC26a6-5′ RACE | GGAAATGTTGATGGCGTAGCCCACG | 1124† |
| SLC26a6-n5′ RACE | GCCACAGCAAACGCATCACCTATCAC | 1096† |
| SLC26a6-3′ RACE | GCCTGTCGCTCCAGACGCAAGTATG | 3245† |
| SLC26a6-n3′ RACE | GTGATAGGTGATGCGTTTGCTGTGGC | 3211† |
| ORF SLC26a6-F | ATGGAGGAGAGGGACGGTTC | 2473 |
| ORF SLC26a6-R | TCACATCTGGGTGGTGCAGG | |
| qPCR SLC26a6-F | CTCTCACTTATTTATACTGTGGTG | 140 |
| qPCR SLC26a6-R | GCAGGTTATGTCTGTAACATGC | |
| EF1α-F | AGGTCATCATCCTGAACCAC | 143 |
| EF1α-R | GTTGTCCTCAAGCTTCTTGC | |
| 18S-F | GCTCGTAGTTGGATCTCGG | 166 |
| 18S-R | GGCCTGCTTTGAACACTC |
| Primer . | Sequence (5′→3′) . | Product size (bp) . |
|---|---|---|
| Eel SLC26a6-F* | TGGTGCGGTTTGGATTTGTG | 544 |
| Eel SLC26a6-R* | ACCAGTTCCTGGTTGCTGTC | |
| SLC26a6-5′ RACE | GGAAATGTTGATGGCGTAGCCCACG | 1124† |
| SLC26a6-n5′ RACE | GCCACAGCAAACGCATCACCTATCAC | 1096† |
| SLC26a6-3′ RACE | GCCTGTCGCTCCAGACGCAAGTATG | 3245† |
| SLC26a6-n3′ RACE | GTGATAGGTGATGCGTTTGCTGTGGC | 3211† |
| ORF SLC26a6-F | ATGGAGGAGAGGGACGGTTC | 2473 |
| ORF SLC26a6-R | TCACATCTGGGTGGTGCAGG | |
| qPCR SLC26a6-F | CTCTCACTTATTTATACTGTGGTG | 140 |
| qPCR SLC26a6-R | GCAGGTTATGTCTGTAACATGC | |
| EF1α-F | AGGTCATCATCCTGAACCAC | 143 |
| EF1α-R | GTTGTCCTCAAGCTTCTTGC | |
| 18S-F | GCTCGTAGTTGGATCTCGG | 166 |
| 18S-R | GGCCTGCTTTGAACACTC |
Primer sequences used for initial cloning of toadfish SLC26a6 fragment,generously provided by Dr Jonathan Wilson, were designed from conserved regions of three eel SLC26a6 aligned sequences
Sizes include 45 bp at 5′ end corresponding to Clontech Universal Primer A sequence. Abbreviations: forward primer (F); reverse primer (R);nested primer (n); open reading frame (ORF)