The effects of the Ca2+ ionophore A23187 and dinitrophenol on the gap junction resistance (Rgi) and the non-junctional resistance (Rnj)
| . | Control (8) . | Leucokinin-VIII (8) . | Leucokinin-VIII (8) A23187 . | Leucokinin-VIII (6) A23187 Dinitrophenol . |
|---|---|---|---|---|
| Vbl1 (mV) | –83.5±3.3 | –103.3±2.1* | –99.3±3.6 | –13.5±4.3* |
| Rinput (kΩ) | 273.7±22.1 | 214.9±9.3* | 193.7±5.8* | 1474.3±191.3* |
| Rgj (kΩ) | 468.0±72.2 | 526.1±76.6 | 30 762.1±6594.6* | |
| Rnj (kΩ) | 578.5±60.9 | 504.0±63.1* | 1483.8±109.2* |
| . | Control (8) . | Leucokinin-VIII (8) . | Leucokinin-VIII (8) A23187 . | Leucokinin-VIII (6) A23187 Dinitrophenol . |
|---|---|---|---|---|
| Vbl1 (mV) | –83.5±3.3 | –103.3±2.1* | –99.3±3.6 | –13.5±4.3* |
| Rinput (kΩ) | 273.7±22.1 | 214.9±9.3* | 193.7±5.8* | 1474.3±191.3* |
| Rgj (kΩ) | 468.0±72.2 | 526.1±76.6 | 30 762.1±6594.6* | |
| Rnj (kΩ) | 578.5±60.9 | 504.0±63.1* | 1483.8±109.2* |
Values are means ± s.e.m. (number of tubule experiments); *significantly different (P<0.05) from the previous treatment by the paired Student's t-test
Vbl1, basolateral membrane voltage of principal cell 1; Rinput, input resistance of cell 1; Rnj, non-junctional resistance
Tubules were first treated with 1 μmol l–1leucokinin-VIII, then with 2 μmol l–1 A23187 in the presence of leucokinin-VIII, and finally with 0.5 mmol l–1dinitrophenol in the presence of leucokinin-VIII and A23187