Measurement of the gap junction resistance Rgj in Malpighian tubules of Aedes aegypti by circuit analysis
| . | Control . | Leucokinin-VIII . |
|---|---|---|
| Vbl1 (mV) | –82.2±2.5 | –100.4±2.7* |
| Iinject (nA) | 168.8±13.5 | 211.5±16.1* |
| Δ Vbl1 (mV) | 40 | 40 |
| Δ Vbl2 (mV) | 20.7±1.2 | 17.0±1.0* |
| Δ Vbl3 (mV) | 11.9±1.0 | 8.3±0.6* |
| Rgj (kΩ) | 431.0±56.5 | |
| Rnj (kΩ) | 546.7±59.3 |
| . | Control . | Leucokinin-VIII . |
|---|---|---|
| Vbl1 (mV) | –82.2±2.5 | –100.4±2.7* |
| Iinject (nA) | 168.8±13.5 | 211.5±16.1* |
| Δ Vbl1 (mV) | 40 | 40 |
| Δ Vbl2 (mV) | 20.7±1.2 | 17.0±1.0* |
| Δ Vbl3 (mV) | 11.9±1.0 | 8.3±0.6* |
| Rgj (kΩ) | 431.0±56.5 | |
| Rnj (kΩ) | 546.7±59.3 |
Values are means ± s.e.m. of 14 experiments; *significantly different (P<0.05) from control by the paired Student's t-test
Vbl, basolateral membrane voltage in principal cells 1,2 and 3; Rgj, gap junction resistance; Rnj, non-junctional resistance
Leucokinin-VIII is used to eliminate electrical coupling of principal cells through the tubule lumen