The Company of Biologists

Table 2.

Cyclic AMP content of Malpighian tubules

	1 min incubation (pmol per tubule set)	10 min incubation (pmol per tubule set)	10 min incubation with IBMX (pmol per tubule set)
Saline	5.7±0.79	7.8±1.09	9.6±1.07
Serotonin (10^–6 mol l^–1)	9.3±0.84^*	9.7±1.04^*	18.0±1.07^*
Rhopr/Dippu-DH₃₁ (10^–8 mol l^–1)	7.6±0.51	6.1±1.16	9.7±0.65
Rhopr/Dippu-DH₃₁ (10^–6 mol l^–1)	5.1±0.81	7.5±0.48	8.3±0.97

	1 min incubation (pmol per tubule set)	10 min incubation (pmol per tubule set)	10 min incubation with IBMX (pmol per tubule set)
Saline	5.7±0.79	7.8±1.09	9.6±1.07
Serotonin (10^–6 mol l^–1)	9.3±0.84^*	9.7±1.04^*	18.0±1.07^*
Rhopr/Dippu-DH₃₁ (10^–8 mol l^–1)	7.6±0.51	6.1±1.16	9.7±0.65
Rhopr/Dippu-DH₃₁ (10^–6 mol l^–1)	5.1±0.81	7.5±0.48	8.3±0.97

IBMX, isobutyl methylxanthine. ^*Significantly different from saline control values (Student's unpaired t test, P<0.05)