The ability of laminarinase and endo-β-1,4-glucanase purified from the midgut gland of G. natalis and C. destructor to hydrolyse carboxymethyl cellulose (endo-β-1,4-glucanase activity), laminarin(laminarinase activity), lichenan (lichenase activity) and cellobiose(β-1,4-glucosidase activity)
| . | Endo-β-1,4-glucanase activity (μmol min–1 mg protein–1) . | Laminarinase activity (μmol min–1 mg protein–1) . | Lichenase activity (μmol min–1 mg protein–1) . | β-1,4-Glucosidase activity (μmol min–1 mg protein–1) . |
|---|---|---|---|---|
| Gecarcoidea natalis | ||||
| Laminarinase | 0.93±0.77 | 15.21±7.41 | 0.07±0.01 | 0.14±0.02 |
| Enzyme activity as a percentage of laminarinase activity | 6.1 | 100 | 0.4 | 0.9 |
| Endo-β-1,4-glucanase | 11.43±1.30 | 2.34±0.37 | 0.52±0.49 | 0.18±0.15 |
| Enzyme activity as a percentage of endo-β-1,4-glucanase activity | 100 | 20.4 | 4.6 | 1.6 |
| Cherax destructor | ||||
| Laminarinase | 5.29 | 18.31 | 1.37 | 0.09 |
| Enzyme activity as a percentage of laminarinase activity | 28.9 | 100 | 7.49 | 0.5 |
| Endo-β-1,4-glucanase | 19.09 | 0.47 | – | 0.14 |
| Enzyme activity as a percentage of endo-β-1,4-glucanase activity | 100 | 2.46 | – | 0.71 |
| . | Endo-β-1,4-glucanase activity (μmol min–1 mg protein–1) . | Laminarinase activity (μmol min–1 mg protein–1) . | Lichenase activity (μmol min–1 mg protein–1) . | β-1,4-Glucosidase activity (μmol min–1 mg protein–1) . |
|---|---|---|---|---|
| Gecarcoidea natalis | ||||
| Laminarinase | 0.93±0.77 | 15.21±7.41 | 0.07±0.01 | 0.14±0.02 |
| Enzyme activity as a percentage of laminarinase activity | 6.1 | 100 | 0.4 | 0.9 |
| Endo-β-1,4-glucanase | 11.43±1.30 | 2.34±0.37 | 0.52±0.49 | 0.18±0.15 |
| Enzyme activity as a percentage of endo-β-1,4-glucanase activity | 100 | 20.4 | 4.6 | 1.6 |
| Cherax destructor | ||||
| Laminarinase | 5.29 | 18.31 | 1.37 | 0.09 |
| Enzyme activity as a percentage of laminarinase activity | 28.9 | 100 | 7.49 | 0.5 |
| Endo-β-1,4-glucanase | 19.09 | 0.47 | – | 0.14 |
| Enzyme activity as a percentage of endo-β-1,4-glucanase activity | 100 | 2.46 | – | 0.71 |
Specific activities of endo-β-1,4-glucanase, laminarinase and lichenase are expressed as μmol of reducing sugars produced min–1 mg protein–1. Specific activity ofβ-1,4-glucosidase is expressed as μmol of glucose produced min–1 mg protein–1. Enzyme activities are also expressed as a percentage of the highest activity