Fraction . | Endo-β-1,4-glucanase specific activity (μmol min–1 mg protein–1) . | Purification factor . | Units of endo-β-1,4-glucanase recovered after each chromatography step . | Percentage yield . |
---|---|---|---|---|
Homogenate | 0.23 | – | 39.7 | – |
Re-dissolved 30–60% ammonium sulphate precipitate | 0.17 | 0.75 | 17.5 | 44.09 |
Combined fractions 1–6 after DEAE anion exchange chromatography | 11.61 | 50.57 | 6.01 | 15.14 |
Combined fractions 21–34 after P-100 gel filtration chromatography | 19.09 | 83.18 | 5.26 | 13.24 |
Fraction . | Endo-β-1,4-glucanase specific activity (μmol min–1 mg protein–1) . | Purification factor . | Units of endo-β-1,4-glucanase recovered after each chromatography step . | Percentage yield . |
---|---|---|---|---|
Homogenate | 0.23 | – | 39.7 | – |
Re-dissolved 30–60% ammonium sulphate precipitate | 0.17 | 0.75 | 17.5 | 44.09 |
Combined fractions 1–6 after DEAE anion exchange chromatography | 11.61 | 50.57 | 6.01 | 15.14 |
Combined fractions 21–34 after P-100 gel filtration chromatography | 19.09 | 83.18 | 5.26 | 13.24 |
Purification factors and percentage yields are respectively expressed as a proportion and percentage of that in the initial homogenate. Units of enzyme are given as μmol min–1