Fraction . | Laminarinase specific activity (μmol min–1 mg protein–1) . | Purification factor . | Units of laminarinase recovered after each chromatography step . | Percentage yield . |
---|---|---|---|---|
Homogenate | 0.17 | – | 30.17 | – |
Re-dissolved 30–60% ammonium sulphate precipitate | 0.23 | 1.3 | 23.08 | 76.48 |
Combined fractions 27–36 after DEAE chromatography | 1.32 | 7.55 | 10.74 | 35.58 |
Combined fractions 60–61 after HIC chromatography | 6.37 | 36.52 | 6.07 | 20.12 |
Combined fractions 47–53 after Mono-Q | 18.31 | 104.94 | 4.47 | 14.82 |
Fraction . | Laminarinase specific activity (μmol min–1 mg protein–1) . | Purification factor . | Units of laminarinase recovered after each chromatography step . | Percentage yield . |
---|---|---|---|---|
Homogenate | 0.17 | – | 30.17 | – |
Re-dissolved 30–60% ammonium sulphate precipitate | 0.23 | 1.3 | 23.08 | 76.48 |
Combined fractions 27–36 after DEAE chromatography | 1.32 | 7.55 | 10.74 | 35.58 |
Combined fractions 60–61 after HIC chromatography | 6.37 | 36.52 | 6.07 | 20.12 |
Combined fractions 47–53 after Mono-Q | 18.31 | 104.94 | 4.47 | 14.82 |
Purification factors and percentage yields are respectively expressed as a proportion and percentage of that in the initial homogenate. Units of enzyme are given as μmol min–1