Specific activity, purification factor, units of enzyme and percentage yield for each step in the purification of endo-β-1,4-glucanase from the midgut gland of G. natalis
| Fraction . | Endo-β-1,4-glucanase specific activity (μmol min–1 mg protein–1) . | Purification factor . | Units of endo-β-1,4-glucanase recovered after each chromatography step . | Percentage yield . |
|---|---|---|---|---|
| Homogenate | 0.023 | 1 | 45.5 | |
| Re-dissolved 30–60% ammonium sulphate precipitate | 0.022 | 1 | 10.6 | 23.4 |
| Combined fractions 56–70 from DEAE anion exchange chromatography | 0.166 | 7.2 | 8.13 | 17.9 |
| Combined fractions 28–43 from P-100 gel filtration chromatography | 12.74 | 552 | 4.64 | 10.2 |
| Fraction . | Endo-β-1,4-glucanase specific activity (μmol min–1 mg protein–1) . | Purification factor . | Units of endo-β-1,4-glucanase recovered after each chromatography step . | Percentage yield . |
|---|---|---|---|---|
| Homogenate | 0.023 | 1 | 45.5 | |
| Re-dissolved 30–60% ammonium sulphate precipitate | 0.022 | 1 | 10.6 | 23.4 |
| Combined fractions 56–70 from DEAE anion exchange chromatography | 0.166 | 7.2 | 8.13 | 17.9 |
| Combined fractions 28–43 from P-100 gel filtration chromatography | 12.74 | 552 | 4.64 | 10.2 |
Purification factors and percentage yields are respectively expressed as a proportion and percentage of that in the initial homogenate. Units of enzyme are given as μmol min–1