Specific activity, purification factor, units of enzyme and percentage yield for each step in the purification of laminarinase from G. natalis
| Fraction . | Laminarinase specific activity (μmol min–1 mg protein–1) . | Purification factor . | Units of laminarinase recovered after each chromatography step . | Percentage yield . |
|---|---|---|---|---|
| Homogenate | 0.096 | 1 | 190 | |
| Re-dissolved 30–60% ammonium sulphate precipitate | 0.077 | 0.8 | 37 | 19.5 |
| Combined fractions 37–55 after DEAE anion exchange chromatography | 0.252 | 2.6 | 22 | 11.7 |
| Combined fractions 52–56 after HIC chromatography | 0.945 | 9.8 | 4.0 | 2.1 |
| Combined fractions 7–21 after P-100 gel filtration chromatography | 7.80 | 80.9 | 9.43 | 4.96 |
| Fraction . | Laminarinase specific activity (μmol min–1 mg protein–1) . | Purification factor . | Units of laminarinase recovered after each chromatography step . | Percentage yield . |
|---|---|---|---|---|
| Homogenate | 0.096 | 1 | 190 | |
| Re-dissolved 30–60% ammonium sulphate precipitate | 0.077 | 0.8 | 37 | 19.5 |
| Combined fractions 37–55 after DEAE anion exchange chromatography | 0.252 | 2.6 | 22 | 11.7 |
| Combined fractions 52–56 after HIC chromatography | 0.945 | 9.8 | 4.0 | 2.1 |
| Combined fractions 7–21 after P-100 gel filtration chromatography | 7.80 | 80.9 | 9.43 | 4.96 |
Purification factors and percentage yields are respectively expressed as a proportion and percentage of that in the initial homogenate. Units of enzyme are given as μmol min–1