Nucleotide sequences of primers used in PCR
| Primer . | Direction . | Sequence (5′–3′) . |
|---|---|---|
| HSP1 | F | AAGGTGGARATCATCATCGCCAAYGAYCARGG |
| HSP2 | F | TAYGTNGCVTTCACNGACAC |
| HSP3 | R | AGGTTGTTGTCCTTGGTCATYGC |
| HSP4 | R | TAGAARTCRATRCCYTCGAASAGACAGTC |
| 5′ Primer | F | ATGGCVAAGGCAMGYGCTGTSGGTAT |
| 3′ Primer | R | TTASTCRACTTCTCRATGGTGGG |
| Pag5 | F | TCCGAGTGATTAACGAGCCAACAGC |
| Pag6 | R | CCAGCCGTCTCCAGACCTAAACATA |
| Pag9 | R | TCAATCGTCAATCACACCATCTAA |
| Pag10 | F | ACACTCGGCCGTTAAAGATCTACT |
| Heb1 | F | AACAGGAAACAAGCGAGCAGTCAGAC |
| Heb3 | R | AGGTCTGGGTCTGCTTTGTGGGAAT |
| UnivP | F/R | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT |
| Nested | F/R | AAGCAGTGGTATCAACGCAGAGT |
| Primer . | Direction . | Sequence (5′–3′) . |
|---|---|---|
| HSP1 | F | AAGGTGGARATCATCATCGCCAAYGAYCARGG |
| HSP2 | F | TAYGTNGCVTTCACNGACAC |
| HSP3 | R | AGGTTGTTGTCCTTGGTCATYGC |
| HSP4 | R | TAGAARTCRATRCCYTCGAASAGACAGTC |
| 5′ Primer | F | ATGGCVAAGGCAMGYGCTGTSGGTAT |
| 3′ Primer | R | TTASTCRACTTCTCRATGGTGGG |
| Pag5 | F | TCCGAGTGATTAACGAGCCAACAGC |
| Pag6 | R | CCAGCCGTCTCCAGACCTAAACATA |
| Pag9 | R | TCAATCGTCAATCACACCATCTAA |
| Pag10 | F | ACACTCGGCCGTTAAAGATCTACT |
| Heb1 | F | AACAGGAAACAAGCGAGCAGTCAGAC |
| Heb3 | R | AGGTCTGGGTCTGCTTTGTGGGAAT |
| UnivP | F/R | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT |
| Nested | F/R | AAGCAGTGGTATCAACGCAGAGT |
Nucleotide codes: R, A/G; Y,C/T; N, A/T/G/C; S, G/C. F, forward; R,reverse