. | Mouse . | . | Sheep . | . | Cow . | . | |||
---|---|---|---|---|---|---|---|---|---|
Phospholipid . | GC . | MS . | GC . | MS . | GC . | MS . | |||
Kidney | |||||||||
16:0 | 12.9±0.2* | 34.5±0.4s | 18.5±0.6* | 25.9±0.4m,c | 20.7±0.5* | 33.4±0.9s | |||
18:0 | 15.8±0.4 | 15.6±0.2s,c | 16.8±0.4 | 18.2±0.1m,c | 12.9±0.2* | 8.8±0.5m,s | |||
18:1 | 7.8±0.1* | 11.7±0.2s,c | 16.0±0.9* | 22.0±0.4m | 15.7±0.3 | 17.8±1.1m | |||
18:2n-6 | 9.5±0.3 | 8.6±0.4c | 10.1±0.7 | 11.2±0.7c | 15.6±2.4 | 17.5±1.3m,s | |||
20:4n-6 | 23.3±0.5* | 18.3±0.3s,c | 13.4±0.7* | 9.8±0.5m | 13.1±0.4* | 9.1±0.3m | |||
22:5n-6 | 1.1±0.3 | 1.6±0.1s,c | – | 3.0±0.2m | – | 2.6±0.2m | |||
22:6n-3 | 16.6±0.6* | 7.8±0.4s,c | 3.1±0.2 | 2.7±0.3m,c | 1.3±0.1 | 1.0±0.1m,s | |||
UI | 249±4* | 161±3s,c | 172±3* | 136±2m,c | 163±5* | 128±1m,s | |||
Σn-6 | 36.5±0.6* | 28.3±0.2s | 24.2±1.1 | 21.6±0.4m,c | 30.6±0.6 | 27.9±1.1s | |||
Σn-3 | 17.7±0.7* | 9.4±0.4c | 14.1±0.5* | 10.1±0.6c | 10.0±2.4 | 6.4±0.6m,s | |||
ΣUNSAT | 71.0±0.6* | 49.8±0.3s,c | 63.4±0.5* | 55.1±0.4m,c | 65.3±0.5* | 56.7±0.5m,s | |||
ΣMUFA | 16.5±0.2* | 12.2±0.2s,c | 24.4±0.8 | 23.4±0.4m | 24.3±0.8 | 22.4±1.2m | |||
ΣPUFA | 54.6±0.8* | 37.6±0.4s,c | 39.0±1.1* | 31.6±0.5m,c | 41.1±1.0* | 34.2±0.7m,s | |||
Brain | |||||||||
16:0 | 21.9±1.6* | 36.5±1.0 | 17.2±0.7* | 35.9±0.6 | 17.4±1.1* | 34.5±0.7 | |||
18:0 | 15.8±0.8* | 19.5±0.6 | 13.0±0.3* | 18.0±0.6 | 14.0±0.8* | 18.0±0.4 | |||
18:1 | 20.2±0.5 | 21.6±0.6c | 21.4±1.0* | 25.5±0.5 | 26.8±2.4 | 28.4±2.0m | |||
20:4n-6 | 10.5±0.7* | 6.4±0.4s,c | 7.3±0.6* | 4.2±0.4m | 6.7±0.5* | 3.6±0.3m | |||
22:6n-3 | 17.7±2.1 | 13.0±0.4c | 21.0±1.2* | 11.3±0.1 | 14.7±2.7 | 9.1±1.3m | |||
UI | 196±15 | 144±5 | 218±7* | 138±3 | 182±13 | 128±4 | |||
Σn-6 | 14.7±1.0* | 7.6±0.5s,c | 12.7±0.8* | 5.8±0.4m | 12.1±0.5* | 5.6±0.4m | |||
Σn-3 | 18.2±1.9 | 13.0±0.4c | 22.8±1.1* | 11.4±0.1 | 16.1±2.6 | 9.1±1.3m | |||
ΣUNSAT | 62.2±2.1* | 43.7±0.5c | 68.1±0.9* | 45.6±0.4 | 68.3±1.8* | 46.6±0.9m | |||
ΣMUFA | 29.2±1.0* | 23.1±0.6s,c | 32.0±1.3 | 28.4±0.8m | 39.5±3.4 | 31.8±2.1m | |||
ΣPUFA | 33.0±3.0 | 20.6±1.0s,c | 36.1±1.5* | 17.2±0.5m | 28.8±2.7* | 14.8±1.3m |
. | Mouse . | . | Sheep . | . | Cow . | . | |||
---|---|---|---|---|---|---|---|---|---|
Phospholipid . | GC . | MS . | GC . | MS . | GC . | MS . | |||
Kidney | |||||||||
16:0 | 12.9±0.2* | 34.5±0.4s | 18.5±0.6* | 25.9±0.4m,c | 20.7±0.5* | 33.4±0.9s | |||
18:0 | 15.8±0.4 | 15.6±0.2s,c | 16.8±0.4 | 18.2±0.1m,c | 12.9±0.2* | 8.8±0.5m,s | |||
18:1 | 7.8±0.1* | 11.7±0.2s,c | 16.0±0.9* | 22.0±0.4m | 15.7±0.3 | 17.8±1.1m | |||
18:2n-6 | 9.5±0.3 | 8.6±0.4c | 10.1±0.7 | 11.2±0.7c | 15.6±2.4 | 17.5±1.3m,s | |||
20:4n-6 | 23.3±0.5* | 18.3±0.3s,c | 13.4±0.7* | 9.8±0.5m | 13.1±0.4* | 9.1±0.3m | |||
22:5n-6 | 1.1±0.3 | 1.6±0.1s,c | – | 3.0±0.2m | – | 2.6±0.2m | |||
22:6n-3 | 16.6±0.6* | 7.8±0.4s,c | 3.1±0.2 | 2.7±0.3m,c | 1.3±0.1 | 1.0±0.1m,s | |||
UI | 249±4* | 161±3s,c | 172±3* | 136±2m,c | 163±5* | 128±1m,s | |||
Σn-6 | 36.5±0.6* | 28.3±0.2s | 24.2±1.1 | 21.6±0.4m,c | 30.6±0.6 | 27.9±1.1s | |||
Σn-3 | 17.7±0.7* | 9.4±0.4c | 14.1±0.5* | 10.1±0.6c | 10.0±2.4 | 6.4±0.6m,s | |||
ΣUNSAT | 71.0±0.6* | 49.8±0.3s,c | 63.4±0.5* | 55.1±0.4m,c | 65.3±0.5* | 56.7±0.5m,s | |||
ΣMUFA | 16.5±0.2* | 12.2±0.2s,c | 24.4±0.8 | 23.4±0.4m | 24.3±0.8 | 22.4±1.2m | |||
ΣPUFA | 54.6±0.8* | 37.6±0.4s,c | 39.0±1.1* | 31.6±0.5m,c | 41.1±1.0* | 34.2±0.7m,s | |||
Brain | |||||||||
16:0 | 21.9±1.6* | 36.5±1.0 | 17.2±0.7* | 35.9±0.6 | 17.4±1.1* | 34.5±0.7 | |||
18:0 | 15.8±0.8* | 19.5±0.6 | 13.0±0.3* | 18.0±0.6 | 14.0±0.8* | 18.0±0.4 | |||
18:1 | 20.2±0.5 | 21.6±0.6c | 21.4±1.0* | 25.5±0.5 | 26.8±2.4 | 28.4±2.0m | |||
20:4n-6 | 10.5±0.7* | 6.4±0.4s,c | 7.3±0.6* | 4.2±0.4m | 6.7±0.5* | 3.6±0.3m | |||
22:6n-3 | 17.7±2.1 | 13.0±0.4c | 21.0±1.2* | 11.3±0.1 | 14.7±2.7 | 9.1±1.3m | |||
UI | 196±15 | 144±5 | 218±7* | 138±3 | 182±13 | 128±4 | |||
Σn-6 | 14.7±1.0* | 7.6±0.5s,c | 12.7±0.8* | 5.8±0.4m | 12.1±0.5* | 5.6±0.4m | |||
Σn-3 | 18.2±1.9 | 13.0±0.4c | 22.8±1.1* | 11.4±0.1 | 16.1±2.6 | 9.1±1.3m | |||
ΣUNSAT | 62.2±2.1* | 43.7±0.5c | 68.1±0.9* | 45.6±0.4 | 68.3±1.8* | 46.6±0.9m | |||
ΣMUFA | 29.2±1.0* | 23.1±0.6s,c | 32.0±1.3 | 28.4±0.8m | 39.5±3.4 | 31.8±2.1m | |||
ΣPUFA | 33.0±3.0 | 20.6±1.0s,c | 36.1±1.5* | 17.2±0.5m | 28.8±2.7* | 14.8±1.3m |
MS, mass spectrometry; GC, gas chromatography [data from Hulbert et al.(Hulbert et al., 2002b)]. For the purpose of calculating Σn-6 and Σn-3 values the minor contributions of 20:3 were designated as predominantly omega-6 and those for 20:5 and 22:5 as omega-3 in kidney and 22:5 designated as predominantly omega-6 in brain based on the results of Hulbert et al. ΣUNSAT, total of unsaturated fatty acids; ΣMUFA, total monounsaturated fatty acids;ΣPUFA, total polyunsaturated fatty acids; Σn-6 and Σn-3,total polyunsaturated omega-6 and omega-3 fatty acids, respectively; UI,unsaturation index (the number double bonds per 100 fatty acid molecules)
Values are mean percentage ± s.e.m. (N=4). Superscript letters in the MS columns indicate significant difference between the values derived for mouse (m), sheep (s) and cow (c) at a minimum of P<0.05 whereas in the GC column the asterisk indicates a significant difference (P<0.05) between measurements made by GC[in the study of Hulbert et al. (Hulbert et al., 2002b)] and to those made by MS (the present study)