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Table 5.

A comparison of the percentage of major fatty acids in kidney and brain of mouse, sheep and cow, as measured by gas chromatography and mass spectrometry

Mouse
Sheep
Cow
PhospholipidGCMSGCMSGCMS
Kidney       
   16:0 12.9±0.2* 34.5±0.4s 18.5±0.6* 25.9±0.4m,c 20.7±0.5* 33.4±0.9s 
   18:0 15.8±0.4 15.6±0.2s,c 16.8±0.4 18.2±0.1m,c 12.9±0.2* 8.8±0.5m,s 
   18:1 7.8±0.1* 11.7±0.2s,c 16.0±0.9* 22.0±0.4m 15.7±0.3 17.8±1.1m 
   18:2n-6 9.5±0.3 8.6±0.4c 10.1±0.7 11.2±0.7c 15.6±2.4 17.5±1.3m,s 
   20:4n-6 23.3±0.5* 18.3±0.3s,c 13.4±0.7* 9.8±0.5m 13.1±0.4* 9.1±0.3m 
   22:5n-6 1.1±0.3 1.6±0.1s,c – 3.0±0.2m – 2.6±0.2m 
   22:6n-3 16.6±0.6* 7.8±0.4s,c 3.1±0.2 2.7±0.3m,c 1.3±0.1 1.0±0.1m,s 
   UI 249±4* 161±3s,c 172±3* 136±2m,c 163±5* 128±1m,s 
   Σn-6 36.5±0.6* 28.3±0.2s 24.2±1.1 21.6±0.4m,c 30.6±0.6 27.9±1.1s 
   Σn-3 17.7±0.7* 9.4±0.4c 14.1±0.5* 10.1±0.6c 10.0±2.4 6.4±0.6m,s 
   ΣUNSAT 71.0±0.6* 49.8±0.3s,c 63.4±0.5* 55.1±0.4m,c 65.3±0.5* 56.7±0.5m,s 
   ΣMUFA 16.5±0.2* 12.2±0.2s,c 24.4±0.8 23.4±0.4m 24.3±0.8 22.4±1.2m 
   ΣPUFA 54.6±0.8* 37.6±0.4s,c 39.0±1.1* 31.6±0.5m,c 41.1±1.0* 34.2±0.7m,s 
Brain       
   16:0 21.9±1.6* 36.5±1.0 17.2±0.7* 35.9±0.6 17.4±1.1* 34.5±0.7 
   18:0 15.8±0.8* 19.5±0.6 13.0±0.3* 18.0±0.6 14.0±0.8* 18.0±0.4 
   18:1 20.2±0.5 21.6±0.6c 21.4±1.0* 25.5±0.5 26.8±2.4 28.4±2.0m 
   20:4n-6 10.5±0.7* 6.4±0.4s,c 7.3±0.6* 4.2±0.4m 6.7±0.5* 3.6±0.3m 
   22:6n-3 17.7±2.1 13.0±0.4c 21.0±1.2* 11.3±0.1 14.7±2.7 9.1±1.3m 
   UI 196±15 144±5 218±7* 138±3 182±13 128±4 
   Σn-6 14.7±1.0* 7.6±0.5s,c 12.7±0.8* 5.8±0.4m 12.1±0.5* 5.6±0.4m 
   Σn-3 18.2±1.9 13.0±0.4c 22.8±1.1* 11.4±0.1 16.1±2.6 9.1±1.3m 
   ΣUNSAT 62.2±2.1* 43.7±0.5c 68.1±0.9* 45.6±0.4 68.3±1.8* 46.6±0.9m 
   ΣMUFA 29.2±1.0* 23.1±0.6s,c 32.0±1.3 28.4±0.8m 39.5±3.4 31.8±2.1m 
   ΣPUFA 33.0±3.0 20.6±1.0s,c 36.1±1.5* 17.2±0.5m 28.8±2.7* 14.8±1.3m 
Mouse
Sheep
Cow
PhospholipidGCMSGCMSGCMS
Kidney       
   16:0 12.9±0.2* 34.5±0.4s 18.5±0.6* 25.9±0.4m,c 20.7±0.5* 33.4±0.9s 
   18:0 15.8±0.4 15.6±0.2s,c 16.8±0.4 18.2±0.1m,c 12.9±0.2* 8.8±0.5m,s 
   18:1 7.8±0.1* 11.7±0.2s,c 16.0±0.9* 22.0±0.4m 15.7±0.3 17.8±1.1m 
   18:2n-6 9.5±0.3 8.6±0.4c 10.1±0.7 11.2±0.7c 15.6±2.4 17.5±1.3m,s 
   20:4n-6 23.3±0.5* 18.3±0.3s,c 13.4±0.7* 9.8±0.5m 13.1±0.4* 9.1±0.3m 
   22:5n-6 1.1±0.3 1.6±0.1s,c – 3.0±0.2m – 2.6±0.2m 
   22:6n-3 16.6±0.6* 7.8±0.4s,c 3.1±0.2 2.7±0.3m,c 1.3±0.1 1.0±0.1m,s 
   UI 249±4* 161±3s,c 172±3* 136±2m,c 163±5* 128±1m,s 
   Σn-6 36.5±0.6* 28.3±0.2s 24.2±1.1 21.6±0.4m,c 30.6±0.6 27.9±1.1s 
   Σn-3 17.7±0.7* 9.4±0.4c 14.1±0.5* 10.1±0.6c 10.0±2.4 6.4±0.6m,s 
   ΣUNSAT 71.0±0.6* 49.8±0.3s,c 63.4±0.5* 55.1±0.4m,c 65.3±0.5* 56.7±0.5m,s 
   ΣMUFA 16.5±0.2* 12.2±0.2s,c 24.4±0.8 23.4±0.4m 24.3±0.8 22.4±1.2m 
   ΣPUFA 54.6±0.8* 37.6±0.4s,c 39.0±1.1* 31.6±0.5m,c 41.1±1.0* 34.2±0.7m,s 
Brain       
   16:0 21.9±1.6* 36.5±1.0 17.2±0.7* 35.9±0.6 17.4±1.1* 34.5±0.7 
   18:0 15.8±0.8* 19.5±0.6 13.0±0.3* 18.0±0.6 14.0±0.8* 18.0±0.4 
   18:1 20.2±0.5 21.6±0.6c 21.4±1.0* 25.5±0.5 26.8±2.4 28.4±2.0m 
   20:4n-6 10.5±0.7* 6.4±0.4s,c 7.3±0.6* 4.2±0.4m 6.7±0.5* 3.6±0.3m 
   22:6n-3 17.7±2.1 13.0±0.4c 21.0±1.2* 11.3±0.1 14.7±2.7 9.1±1.3m 
   UI 196±15 144±5 218±7* 138±3 182±13 128±4 
   Σn-6 14.7±1.0* 7.6±0.5s,c 12.7±0.8* 5.8±0.4m 12.1±0.5* 5.6±0.4m 
   Σn-3 18.2±1.9 13.0±0.4c 22.8±1.1* 11.4±0.1 16.1±2.6 9.1±1.3m 
   ΣUNSAT 62.2±2.1* 43.7±0.5c 68.1±0.9* 45.6±0.4 68.3±1.8* 46.6±0.9m 
   ΣMUFA 29.2±1.0* 23.1±0.6s,c 32.0±1.3 28.4±0.8m 39.5±3.4 31.8±2.1m 
   ΣPUFA 33.0±3.0 20.6±1.0s,c 36.1±1.5* 17.2±0.5m 28.8±2.7* 14.8±1.3m 

MS, mass spectrometry; GC, gas chromatography [data from Hulbert et al.(Hulbert et al., 2002b)]. For the purpose of calculating Σn-6 and Σn-3 values the minor contributions of 20:3 were designated as predominantly omega-6 and those for 20:5 and 22:5 as omega-3 in kidney and 22:5 designated as predominantly omega-6 in brain based on the results of Hulbert et al. ΣUNSAT, total of unsaturated fatty acids; ΣMUFA, total monounsaturated fatty acids;ΣPUFA, total polyunsaturated fatty acids; Σn-6 and Σn-3,total polyunsaturated omega-6 and omega-3 fatty acids, respectively; UI,unsaturation index (the number double bonds per 100 fatty acid molecules)

Values are mean percentage ± s.e.m. (N=4). Superscript letters in the MS columns indicate significant difference between the values derived for mouse (m), sheep (s) and cow (c) at a minimum of P<0.05 whereas in the GC column the asterisk indicates a significant difference (P<0.05) between measurements made by GC[in the study of Hulbert et al. (Hulbert et al., 2002b)] and to those made by MS (the present study)

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