Identification of Tetilla mutabilis protein spots after 2DGE and tryptic in-gel digestion by MALDI-TOF/TOF MS
| Spot ID . | Protein name . | Mascot score (PSD, CID) . | MSBLASTP2 score (PSD, CID) . |
|---|---|---|---|
| 130 | – | – | – |
| 177 | Heat shock protein 70 | –, 53 | –, 106 |
| 228 | Calreticulin | 74, 62 | 191, 216 |
| 240 | – | – | – |
| 275 | – | – | – |
| 276 | Hypothetical protein | –, – | 134, – |
| 306 | Catalase | 91, 88 | 321, 294 |
| 331 | Flagellar basal body rod protein | –, – | –, 65 |
| 341 | Catalase | –, – | –, 209 |
| 350 | – | – | – |
| 360 | – | – | – |
| 409 | Catalase | 68, 56 | –, 273 |
| 411 | Putative thiolase | –, 63 | –, 59 |
| 423 | HSP70-like protein | –, – | 106, – |
| 478 | Gelsolin | 89, 82 | 143, 157 |
| 490 | Serine hydroxymethyl transferase | 94, 88 | 181, 69 |
| 598 | Actin | 360, 316 | 618, 669 |
| 599 | Actin | 521, 618 | 607, 615 |
| 675 | Actin | –, 248 | 348, 341 |
| 689 | – | – | – |
| 724 | Actin | 524, 508 | –, 457 |
| 733 | Glyceraldehyde-3-phosphate dehydrogenase | 153, 150 | 107, 108 |
| 780 | – | – | – |
| 833 | Catalase | 75, – | 222, 186 |
| 949 | – | – | – |
| 1011 | Actin | 227, 203 | 285, 264 |
| 1015 | Polyubiquitin | –, 67 | –, 67 |
| 1114 | Hypothetical protein | –, – | 123, 105 |
| 1135 | – | – | – |
| 1142 | Peroxiredoxin | 108, 73 | 155, 189 |
| 1224 | Tropomyosin | 53, – | 205, 106 |
| 1362 | – | – | – |
| 1368 | Peptidyl prolyl isomerase A | 192, 136 | 116, – |
| 1440 | – | – | – |
| 1461 | Nucleoside diphosphate kinase | 58, – | 65, 65 |
| 1490 | Nucleoside diphosphate kinase | –, – | 133, 171 |
| Spot ID . | Protein name . | Mascot score (PSD, CID) . | MSBLASTP2 score (PSD, CID) . |
|---|---|---|---|
| 130 | – | – | – |
| 177 | Heat shock protein 70 | –, 53 | –, 106 |
| 228 | Calreticulin | 74, 62 | 191, 216 |
| 240 | – | – | – |
| 275 | – | – | – |
| 276 | Hypothetical protein | –, – | 134, – |
| 306 | Catalase | 91, 88 | 321, 294 |
| 331 | Flagellar basal body rod protein | –, – | –, 65 |
| 341 | Catalase | –, – | –, 209 |
| 350 | – | – | – |
| 360 | – | – | – |
| 409 | Catalase | 68, 56 | –, 273 |
| 411 | Putative thiolase | –, 63 | –, 59 |
| 423 | HSP70-like protein | –, – | 106, – |
| 478 | Gelsolin | 89, 82 | 143, 157 |
| 490 | Serine hydroxymethyl transferase | 94, 88 | 181, 69 |
| 598 | Actin | 360, 316 | 618, 669 |
| 599 | Actin | 521, 618 | 607, 615 |
| 675 | Actin | –, 248 | 348, 341 |
| 689 | – | – | – |
| 724 | Actin | 524, 508 | –, 457 |
| 733 | Glyceraldehyde-3-phosphate dehydrogenase | 153, 150 | 107, 108 |
| 780 | – | – | – |
| 833 | Catalase | 75, – | 222, 186 |
| 949 | – | – | – |
| 1011 | Actin | 227, 203 | 285, 264 |
| 1015 | Polyubiquitin | –, 67 | –, 67 |
| 1114 | Hypothetical protein | –, – | 123, 105 |
| 1135 | – | – | – |
| 1142 | Peroxiredoxin | 108, 73 | 155, 189 |
| 1224 | Tropomyosin | 53, – | 205, 106 |
| 1362 | – | – | – |
| 1368 | Peptidyl prolyl isomerase A | 192, 136 | 116, – |
| 1440 | – | – | – |
| 1461 | Nucleoside diphosphate kinase | 58, – | 65, 65 |
| 1490 | Nucleoside diphosphate kinase | –, – | 133, 171 |
Spot ID values correspond to the labels of protein spots in Fig. 2. Scores are based on searches against non-redundant NCBI databases and a 50 p.p.m. threshold for mass accuracy