Depolarization-induced changes in immunolabeling intensity
| Species . | Tissue/label . | Control . | High K+ . | Change (%) . | P value . |
|---|---|---|---|---|---|
| Cancer borealis | AMC/SIFa | 169.8±5.5 | 164.5±6.6 | -3.1±1.9 | 0.0088 |
| PMC/TRP | 158.8±8.5 | 150.2±7.1 | -5.4±1.7 | 0.0009 | |
| Cancer productus | AMC/SIFa | 171.8±6.3 | 165.0±6.0 | -4.0±0.6 | <0.0001 |
| PMC/TRP | 168.7±4.6 | 160.2±5.0 | -5.0±2.3 | 0.0032 | |
| Cancer magister | AMC/SIFa | 164.7±9.3 | 158.3±7.6 | -3.8±2.2 | 0.0083 |
| PMC/TRP | 161.7±8.6 | 155.0±9.1 | -4.1±0.9 | <0.0001 |
| Species . | Tissue/label . | Control . | High K+ . | Change (%) . | P value . |
|---|---|---|---|---|---|
| Cancer borealis | AMC/SIFa | 169.8±5.5 | 164.5±6.6 | -3.1±1.9 | 0.0088 |
| PMC/TRP | 158.8±8.5 | 150.2±7.1 | -5.4±1.7 | 0.0009 | |
| Cancer productus | AMC/SIFa | 171.8±6.3 | 165.0±6.0 | -4.0±0.6 | <0.0001 |
| PMC/TRP | 168.7±4.6 | 160.2±5.0 | -5.0±2.3 | 0.0032 | |
| Cancer magister | AMC/SIFa | 164.7±9.3 | 158.3±7.6 | -3.8±2.2 | 0.0083 |
| PMC/TRP | 161.7±8.6 | 155.0±9.1 | -4.1±0.9 | <0.0001 |
Paired anterior midgut caeca (AMCs) and the single posterior midgut caecum(PMC) from six individual crabs were isolated, and each PMC was divided into two pieces. After 1 h of incubation in control saline, the saline surrounding one member of each tissue pair was replaced with control saline, whereas the other was replaced with high-K+ saline. After 1 h, all tissues were simultaneously immunoprocessed for either SIFamide (SIFa)- or substance P(TRP)-like labeling. Values shown are the mean pixel values for each of the six preparations. Paired two-tailed Student's t-tests were used to compare labeling intensity in control and high-K+ saline(P value). No statistically significant differences were seen in C. borealis tissue pairs when both AMCs or PMC sections were incubated in the same saline (N=6 pairs per tissue and saline; data not shown).