Table 1.

Intrinsic membrane properties of RPeD1 neurons in semi-intact brain preparations

Intrinsic membrane propertiesPWCEBCP
Activity (spikes per 10 min) 94.46±15.96 23.06±5.70 83.20±6.94 <0.01 
Total sample frequency (Hz) 0.16±0.02 0.058±0.012 0.12±0.08 <0.01 
Resting membrane potential (mV) –63.36±1.24 –61.27±1.16 –62.57±1.55 NSD 
Mean spikes per burst 8.91±1.76 2.02±0.38 8.57±1.87 <0.01 
Burst frequency (Hz) 1.55±0.267 1.11±0.14 1.91±0.18 NSD 
After-hyperpolarization (mV) –5.81±1.52 –5.78±0.11 –5.44±1.67 NSD 
Spike amplitude (mV) 83.82±1.18 85.43±1.61 83.88±1.46 NSD 
Burst duration (s) 12.64±2.86 4.52±0.77 9.78±1.22 <0.01 
Intrinsic membrane propertiesPWCEBCP
Activity (spikes per 10 min) 94.46±15.96 23.06±5.70 83.20±6.94 <0.01 
Total sample frequency (Hz) 0.16±0.02 0.058±0.012 0.12±0.08 <0.01 
Resting membrane potential (mV) –63.36±1.24 –61.27±1.16 –62.57±1.55 NSD 
Mean spikes per burst 8.91±1.76 2.02±0.38 8.57±1.87 <0.01 
Burst frequency (Hz) 1.55±0.267 1.11±0.14 1.91±0.18 NSD 
After-hyperpolarization (mV) –5.81±1.52 –5.78±0.11 –5.44±1.67 NSD 
Spike amplitude (mV) 83.82±1.18 85.43±1.61 83.88±1.46 NSD 
Burst duration (s) 12.64±2.86 4.52±0.77 9.78±1.22 <0.01 

Values for pond water (PW), crayfish effluent (CE) and boiled CE (BC) are means ± s.e.m. P<0.01 indicates that CE treatment is significantly different from both PW and BC control groups, and PW and BC are not significantly different from each other. NSD, no significant difference.(All data represent raw non-transformed data.)

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