. | . | PL/ST . | . | Unsaturation . | . | PUFA . | . | 22:6n-3 . | . | 20:5n-3 . | . | 20:4n-6 . | . | 22:2 NMI . | . | 20:2 NMI . | . | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Source of variation . | d.f. . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | |||||||||||||||||
Main plot analysis | ||||||||||||||||||||||||||||||||||
T°C | 2 | 1.4 | 0.373 | 1.1 | 0.446 | 0.4 | 0.677 | 2.6 | 0.225 | 2.1 | 0.264 | 1.6 | 0.343 | 1.4 | 0.372 | 2.6 | 0.222 | |||||||||||||||||
Error A | 3 | |||||||||||||||||||||||||||||||||
Subplot analysis | ||||||||||||||||||||||||||||||||||
Sp. | 1 | 73.2 | 0.003 | 238.8 | 0.001 | 625.4 | 0.001 | 6.0 | 0.090 | 161.6 | 0.001 | 0.4 | 0.588 | 470.7 | <0.001 | 1522.1 | <0.001 | |||||||||||||||||
T°C×Sp. | 2 | 0.0 | 0.967 | 0.5 | 0.644 | 0.5 | 0.652 | 0.6 | 0.621 | 1.5 | 0.356 | 0.8 | 0.517 | 0.8 | 0.517 | 2.0 | 0.280 | |||||||||||||||||
Error B | 3 | |||||||||||||||||||||||||||||||||
Sub-subplot analysis | ||||||||||||||||||||||||||||||||||
Tissue | 1 | 103.2 | <0.001 | 7.6 | 0.008 | 151.7 | <0.001 | 24.6 | <0.001 | 165.4 | <0.001 | 221.9 | <0.001 | 162.0 | <0.001 | 150.5 | <0.001 | |||||||||||||||||
T°C×Tissue | 2 | 1.2 | 0.313 | 0.3 | 0.710 | 0.3 | 0.723 | 0.4 | 0.673 | 1.0 | 0.376 | 0.1 | 0.934 | 0.4 | 0.668 | 0.9 | 0.432 | |||||||||||||||||
Species×Tissue | 1 | 0.1 | 0.733 | 6.2 | 0.016 | 0.0 | 0.973 | 1.0 | 0.311 | 73.3 | <0.001 | 2.0 | 0.161 | 2.2 | 0.140 | 224.0 | <0.001 | |||||||||||||||||
T°C×Species×Tissue | 2 | 0.6 | 0.537 | 0.2 | 0.807 | 0.4 | 0.652 | 0.1 | 0.932 | 1.0 | 0.375 | 0.3 | 0.758 | 0.5 | 0.585 | 0.6 | 0.573 | |||||||||||||||||
Date | 4 | 10.0 | <0.001 | 60.6 | <0.001 | 31.4 | <0.001 | 55.6 | <0.001 | 122.4 | <0.001 | 88.4 | <0.001 | 4.1 | 0.006 | 1.2 | 0.313 | |||||||||||||||||
T°C×Date | 8 | 0.3 | 0.965 | 1.1 | 0.379 | 0.5 | 0.845 | 1.1 | 0.356 | 2.1 | 0.049 | 0.6 | 0.778 | 1.0 | 0.464 | 1.0 | 0.460 | |||||||||||||||||
Species×Data | 4 | 1.1 | 0.348 | 0.8 | 0.550 | 0.1 | 0.973 | 2.1 | 0.095 | 7.7 | <0.001 | 26.6 | <0.001 | 5.1 | 0.002 | 1.8 | 0.136 | |||||||||||||||||
T°C×Species×Date | 8 | 0.4 | 0.921 | 0.7 | 0.715 | 0.5 | 0.823 | 1.1 | 0.383 | 1.4 | 0.209 | 2.6 | 0.017 | 1.9 | 0.083 | 1.4 | 0.234 | |||||||||||||||||
Tissue×Date | 4 | 1.0 | 0.402 | 4.5 | 0.003 | 6.4 | 0.001 | 2.1 | 0.100 | 3.0 | 0.029 | 2.5 | 0.053 | 2.3 | 0.075 | 1.0 | 0.431 | |||||||||||||||||
T°C×Tissue×Date | 8 | 1.8 | 0.107 | 1.0 | 0.448 | 1.1 | 0.437 | 1.0 | 0.479 | 0.7 | 0.681 | 1.2 | 0.348 | 1.6 | 0.139 | 1.1 | 0.358 | |||||||||||||||||
Species×Tissue×Date | 4 | 1.0 | 0.412 | 0.9 | 0.492 | 0.6 | 0.639 | 0.4 | 0.803 | 1.0 | 0.413 | 1.3 | 0.295 | 0.3 | 0.869 | 2.4 | 0.064 | |||||||||||||||||
T°C×Species×Tissue×Date | 8 | 1.6 | 0.157 | 0.7 | 0.662 | 0.8 | 0.576 | 0.7 | 0.721 | 1.3 | 0.246 | 1.3 | 0.268 | 1.0 | 0.433 | 0.2 | 1.340 | |||||||||||||||||
Error C 54 | ||||||||||||||||||||||||||||||||||
Error A: Tank (T°C) | ||||||||||||||||||||||||||||||||||
Error B: Species×Tank (T°C) | ||||||||||||||||||||||||||||||||||
Error C: Tissue×Tank (T°C), d.f.=3; Species×Tissue×Tank(T°C), d.f.=3; Date×Tank (T°C), d.f.=12;Species×Date×Tank (T°C), d.f.=12; Tissue×Date×Tank(T°C), d.f.=12; Species×Tissue×Date×Tank (T°C),d.f.=12 |
. | . | PL/ST . | . | Unsaturation . | . | PUFA . | . | 22:6n-3 . | . | 20:5n-3 . | . | 20:4n-6 . | . | 22:2 NMI . | . | 20:2 NMI . | . | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Source of variation . | d.f. . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | |||||||||||||||||
Main plot analysis | ||||||||||||||||||||||||||||||||||
T°C | 2 | 1.4 | 0.373 | 1.1 | 0.446 | 0.4 | 0.677 | 2.6 | 0.225 | 2.1 | 0.264 | 1.6 | 0.343 | 1.4 | 0.372 | 2.6 | 0.222 | |||||||||||||||||
Error A | 3 | |||||||||||||||||||||||||||||||||
Subplot analysis | ||||||||||||||||||||||||||||||||||
Sp. | 1 | 73.2 | 0.003 | 238.8 | 0.001 | 625.4 | 0.001 | 6.0 | 0.090 | 161.6 | 0.001 | 0.4 | 0.588 | 470.7 | <0.001 | 1522.1 | <0.001 | |||||||||||||||||
T°C×Sp. | 2 | 0.0 | 0.967 | 0.5 | 0.644 | 0.5 | 0.652 | 0.6 | 0.621 | 1.5 | 0.356 | 0.8 | 0.517 | 0.8 | 0.517 | 2.0 | 0.280 | |||||||||||||||||
Error B | 3 | |||||||||||||||||||||||||||||||||
Sub-subplot analysis | ||||||||||||||||||||||||||||||||||
Tissue | 1 | 103.2 | <0.001 | 7.6 | 0.008 | 151.7 | <0.001 | 24.6 | <0.001 | 165.4 | <0.001 | 221.9 | <0.001 | 162.0 | <0.001 | 150.5 | <0.001 | |||||||||||||||||
T°C×Tissue | 2 | 1.2 | 0.313 | 0.3 | 0.710 | 0.3 | 0.723 | 0.4 | 0.673 | 1.0 | 0.376 | 0.1 | 0.934 | 0.4 | 0.668 | 0.9 | 0.432 | |||||||||||||||||
Species×Tissue | 1 | 0.1 | 0.733 | 6.2 | 0.016 | 0.0 | 0.973 | 1.0 | 0.311 | 73.3 | <0.001 | 2.0 | 0.161 | 2.2 | 0.140 | 224.0 | <0.001 | |||||||||||||||||
T°C×Species×Tissue | 2 | 0.6 | 0.537 | 0.2 | 0.807 | 0.4 | 0.652 | 0.1 | 0.932 | 1.0 | 0.375 | 0.3 | 0.758 | 0.5 | 0.585 | 0.6 | 0.573 | |||||||||||||||||
Date | 4 | 10.0 | <0.001 | 60.6 | <0.001 | 31.4 | <0.001 | 55.6 | <0.001 | 122.4 | <0.001 | 88.4 | <0.001 | 4.1 | 0.006 | 1.2 | 0.313 | |||||||||||||||||
T°C×Date | 8 | 0.3 | 0.965 | 1.1 | 0.379 | 0.5 | 0.845 | 1.1 | 0.356 | 2.1 | 0.049 | 0.6 | 0.778 | 1.0 | 0.464 | 1.0 | 0.460 | |||||||||||||||||
Species×Data | 4 | 1.1 | 0.348 | 0.8 | 0.550 | 0.1 | 0.973 | 2.1 | 0.095 | 7.7 | <0.001 | 26.6 | <0.001 | 5.1 | 0.002 | 1.8 | 0.136 | |||||||||||||||||
T°C×Species×Date | 8 | 0.4 | 0.921 | 0.7 | 0.715 | 0.5 | 0.823 | 1.1 | 0.383 | 1.4 | 0.209 | 2.6 | 0.017 | 1.9 | 0.083 | 1.4 | 0.234 | |||||||||||||||||
Tissue×Date | 4 | 1.0 | 0.402 | 4.5 | 0.003 | 6.4 | 0.001 | 2.1 | 0.100 | 3.0 | 0.029 | 2.5 | 0.053 | 2.3 | 0.075 | 1.0 | 0.431 | |||||||||||||||||
T°C×Tissue×Date | 8 | 1.8 | 0.107 | 1.0 | 0.448 | 1.1 | 0.437 | 1.0 | 0.479 | 0.7 | 0.681 | 1.2 | 0.348 | 1.6 | 0.139 | 1.1 | 0.358 | |||||||||||||||||
Species×Tissue×Date | 4 | 1.0 | 0.412 | 0.9 | 0.492 | 0.6 | 0.639 | 0.4 | 0.803 | 1.0 | 0.413 | 1.3 | 0.295 | 0.3 | 0.869 | 2.4 | 0.064 | |||||||||||||||||
T°C×Species×Tissue×Date | 8 | 1.6 | 0.157 | 0.7 | 0.662 | 0.8 | 0.576 | 0.7 | 0.721 | 1.3 | 0.246 | 1.3 | 0.268 | 1.0 | 0.433 | 0.2 | 1.340 | |||||||||||||||||
Error C 54 | ||||||||||||||||||||||||||||||||||
Error A: Tank (T°C) | ||||||||||||||||||||||||||||||||||
Error B: Species×Tank (T°C) | ||||||||||||||||||||||||||||||||||
Error C: Tissue×Tank (T°C), d.f.=3; Species×Tissue×Tank(T°C), d.f.=3; Date×Tank (T°C), d.f.=12;Species×Date×Tank (T°C), d.f.=12; Tissue×Date×Tank(T°C), d.f.=12; Species×Tissue×Date×Tank (T°C),d.f.=12 |
PL/ST, phospholipid to sterol ratio
Independent variables were overwintering temperature (T°C; 0°C,4°C and 9°C), bivalve species (mussel and oyster), tissue (gills and digestive glands) and sampling date (31 January, 14 February, 12 April, 4 May and 8 June)
Significant probabilities are in bold. Data of PL/ST, 20:2 and 22:2 NMI were log+1 transformed