Summary of the split-split plot three-way ANOVAs on the effect of overwintering temperature, bivalve species and sampling date on concentrations of triglyceride, unsaturation index, total PUFA and major individual PUFA of neutral lipids in digestive glands
| . | . | Triglyceride . | . | Unsaturation . | . | PUFA . | . | 22:6n-3 . | . | 20:5n-3 . | . | 18:4n-3 . | . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Source of variation . | d.f. . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | |||||||||||||
| Main plot analysis | ||||||||||||||||||||||||||
| T°C | 2 | 0.3 | 0.749 | 0.2 | 0.813 | 0.1 | 0.917 | 0.7 | 0.564 | 0.5 | 0.671 | 0.4 | 0.699 | |||||||||||||
| Error A | 3 | |||||||||||||||||||||||||
| Subplot analysis | ||||||||||||||||||||||||||
| Sp. | 1 | 13.8 | 0.034 | 74.0 | 0.003 | 14.2 | 0.033 | 8.1 | 0.065 | 89.6 | 0.002 | 2.1 | 0.245 | |||||||||||||
| T°C × Sp. | 2 | 2.5 | 0.227 | 2.4 | 0.241 | 1.8 | 0.309 | 3.3 | 0.176 | 0.2 | 0.850 | 2.7 | 0.214 | |||||||||||||
| Error B | 3 | |||||||||||||||||||||||||
| Sub-subplot analysis | ||||||||||||||||||||||||||
| Date | 4 | 4.1 | 0.012 | 18.1 | <0.001 | 17.0 | <0.001 | 11.7 | <0.001 | 28.9 | <0.001 | 5.3 | 0.003 | |||||||||||||
| T°C × Date | 8 | 2.3 | 0.052 | 1.7 | 0.150 | 1.8 | 0.138 | 2.1 | 0.074 | 0.9 | 0.540 | 2.4 | 0.046 | |||||||||||||
| Sp. × Date | 4 | 3.3 | 0.028 | 6.5 | 0.001 | 5.5 | 0.003 | 1.5 | 0.223 | 13.7 | <0.001 | 6.1 | 0.002 | |||||||||||||
| T°C × Sp. × Date | 8 | 1.0 | 0.438 | 0.6 | 0.800 | 0.4 | 0.917 | 0.6 | 0.786 | 0.9 | 0.560 | 1.0 | 0.490 | |||||||||||||
| Error C | 24 | |||||||||||||||||||||||||
| Error A: Tank (T°C) | ||||||||||||||||||||||||||
| Error B: Sp. × Tank (T°C) | ||||||||||||||||||||||||||
| Error C: Date × Tank (T°C), d.f.=12; Sp. × Date × Tank(T°C), d.f.=12 | ||||||||||||||||||||||||||
| . | . | Triglyceride . | . | Unsaturation . | . | PUFA . | . | 22:6n-3 . | . | 20:5n-3 . | . | 18:4n-3 . | . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Source of variation . | d.f. . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | F . | P . | |||||||||||||
| Main plot analysis | ||||||||||||||||||||||||||
| T°C | 2 | 0.3 | 0.749 | 0.2 | 0.813 | 0.1 | 0.917 | 0.7 | 0.564 | 0.5 | 0.671 | 0.4 | 0.699 | |||||||||||||
| Error A | 3 | |||||||||||||||||||||||||
| Subplot analysis | ||||||||||||||||||||||||||
| Sp. | 1 | 13.8 | 0.034 | 74.0 | 0.003 | 14.2 | 0.033 | 8.1 | 0.065 | 89.6 | 0.002 | 2.1 | 0.245 | |||||||||||||
| T°C × Sp. | 2 | 2.5 | 0.227 | 2.4 | 0.241 | 1.8 | 0.309 | 3.3 | 0.176 | 0.2 | 0.850 | 2.7 | 0.214 | |||||||||||||
| Error B | 3 | |||||||||||||||||||||||||
| Sub-subplot analysis | ||||||||||||||||||||||||||
| Date | 4 | 4.1 | 0.012 | 18.1 | <0.001 | 17.0 | <0.001 | 11.7 | <0.001 | 28.9 | <0.001 | 5.3 | 0.003 | |||||||||||||
| T°C × Date | 8 | 2.3 | 0.052 | 1.7 | 0.150 | 1.8 | 0.138 | 2.1 | 0.074 | 0.9 | 0.540 | 2.4 | 0.046 | |||||||||||||
| Sp. × Date | 4 | 3.3 | 0.028 | 6.5 | 0.001 | 5.5 | 0.003 | 1.5 | 0.223 | 13.7 | <0.001 | 6.1 | 0.002 | |||||||||||||
| T°C × Sp. × Date | 8 | 1.0 | 0.438 | 0.6 | 0.800 | 0.4 | 0.917 | 0.6 | 0.786 | 0.9 | 0.560 | 1.0 | 0.490 | |||||||||||||
| Error C | 24 | |||||||||||||||||||||||||
| Error A: Tank (T°C) | ||||||||||||||||||||||||||
| Error B: Sp. × Tank (T°C) | ||||||||||||||||||||||||||
| Error C: Date × Tank (T°C), d.f.=12; Sp. × Date × Tank(T°C), d.f.=12 | ||||||||||||||||||||||||||
Independent variables were overwintering temperature (T°C; 0°C,4°C and 9°C), bivalve species (Sp.; mussel and oyster) and Date (31 January, 14 February, 12 April, 4 May and 8 June)
Significant probabilities are in bold. Triglyceride data were log transformed and 18:4n-3 data were 1/square root(x) transformed