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Table 3.

Summary of the split-split plot three-way ANOVAs on the effect of overwintering temperature, bivalve species and sampling date on concentrations of triglyceride, unsaturation index, total PUFA and major individual PUFA of neutral lipids in digestive glands

Triglyceride
Unsaturation
PUFA
22:6n-3
20:5n-3
18:4n-3
Source of variationd.f.FPFPFPFPFPFP
Main plot analysis              
   T°C 0.3 0.749 0.2 0.813 0.1 0.917 0.7 0.564 0.5 0.671 0.4 0.699 
   Error A             
Subplot analysis              
   Sp. 13.8 0.034 74.0 0.003 14.2 0.033 8.1 0.065 89.6 0.002 2.1 0.245 
   T°C × Sp. 2.5 0.227 2.4 0.241 1.8 0.309 3.3 0.176 0.2 0.850 2.7 0.214 
   Error B             
Sub-subplot analysis              
   Date 4.1 0.012 18.1 <0.001 17.0 <0.001 11.7 <0.001 28.9 <0.001 5.3 0.003 
   T°C × Date 2.3 0.052 1.7 0.150 1.8 0.138 2.1 0.074 0.9 0.540 2.4 0.046 
   Sp. × Date 3.3 0.028 6.5 0.001 5.5 0.003 1.5 0.223 13.7 <0.001 6.1 0.002 
   T°C × Sp. × Date 1.0 0.438 0.6 0.800 0.4 0.917 0.6 0.786 0.9 0.560 1.0 0.490 
   Error C 24             
Error A: Tank (T°C)              
Error B: Sp. × Tank (T°C)              
Error C: Date × Tank (T°C), d.f.=12; Sp. × Date × Tank(T°C), d.f.=12              
Triglyceride
Unsaturation
PUFA
22:6n-3
20:5n-3
18:4n-3
Source of variationd.f.FPFPFPFPFPFP
Main plot analysis              
   T°C 0.3 0.749 0.2 0.813 0.1 0.917 0.7 0.564 0.5 0.671 0.4 0.699 
   Error A             
Subplot analysis              
   Sp. 13.8 0.034 74.0 0.003 14.2 0.033 8.1 0.065 89.6 0.002 2.1 0.245 
   T°C × Sp. 2.5 0.227 2.4 0.241 1.8 0.309 3.3 0.176 0.2 0.850 2.7 0.214 
   Error B             
Sub-subplot analysis              
   Date 4.1 0.012 18.1 <0.001 17.0 <0.001 11.7 <0.001 28.9 <0.001 5.3 0.003 
   T°C × Date 2.3 0.052 1.7 0.150 1.8 0.138 2.1 0.074 0.9 0.540 2.4 0.046 
   Sp. × Date 3.3 0.028 6.5 0.001 5.5 0.003 1.5 0.223 13.7 <0.001 6.1 0.002 
   T°C × Sp. × Date 1.0 0.438 0.6 0.800 0.4 0.917 0.6 0.786 0.9 0.560 1.0 0.490 
   Error C 24             
Error A: Tank (T°C)              
Error B: Sp. × Tank (T°C)              
Error C: Date × Tank (T°C), d.f.=12; Sp. × Date × Tank(T°C), d.f.=12              

Independent variables were overwintering temperature (T°C; 0°C,4°C and 9°C), bivalve species (Sp.; mussel and oyster) and Date (31 January, 14 February, 12 April, 4 May and 8 June)

Significant probabilities are in bold. Triglyceride data were log transformed and 18:4n-3 data were 1/square root(x) transformed

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