Table 2.

Exon–intron boundary sequences and splice site strength parameters of T. rubripes FoxK1-α

ExonSize (bp)Donor (3′ boundary) SequenceScoreAcceptor (5′ boundary) SequenceScore
435 CCAGAGAgtaagtgc 0.99   
216 CCATCAGgtgagaag 0.99 ttttctagGTGTGTG 0.99 
199 CCCAAAGgtgaagtc 0.54* tccttcagCGTTCCC 0.63* 
147 CTGGCAGgtgggccc 0.64* ttttccagGATGAGT 0.99 
194 CCTCCAGgtgagtgg 0.99 cttttcagAACTCCA 0.89 
173 GCTCCAGgtgagtct 1.00 ttctacagAAGCGCC 0.99 
312   ctccgtagGATCTCC 0.92 
ExonSize (bp)Donor (3′ boundary) SequenceScoreAcceptor (5′ boundary) SequenceScore
435 CCAGAGAgtaagtgc 0.99   
216 CCATCAGgtgagaag 0.99 ttttctagGTGTGTG 0.99 
199 CCCAAAGgtgaagtc 0.54* tccttcagCGTTCCC 0.63* 
147 CTGGCAGgtgggccc 0.64* ttttccagGATGAGT 0.99 
194 CCTCCAGgtgagtgg 0.99 cttttcagAACTCCA 0.89 
173 GCTCCAGgtgagtct 1.00 ttctacagAAGCGCC 0.99 
312   ctccgtagGATCTCC 0.92 

Exonic and intronic sequences are represented in upper and lower case,respectively. Exon–intron boundaries are highlighted in bold. *Splice site scores that are significantly lower than the mean value (P=0.02)

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