List of primer pairs used to amplify FoxK1 from Takifugu rubripes
| Primer pair . | Forward primer (5′→3′) . | Reverse primer (5′→3′) . | Amplicon size (bp) . |
|---|---|---|---|
| cDNA-FoxK1 | GGACGACACTGGAGCAAGA | GGGGCTCATTTTTGATCAAG | 1676 |
| ISH-FoxK1-α | GAGTCCAAGCCACCTTATTC | GGGGCTCATTTTTGATCAAG | 823 |
| ISH-FoxK1-γ | GAGTCCAAGCCACCTTATTC | CTGGAGCGCTCTGGGAGTAC | 703 |
| ISH-FoxK1-δ | GAGTCCAAGCCACCTTATTC | CTGGAGCGCTCTGGGAGTAC | 940 |
| qPCR-FoxK1-α | GCTGGCAGAACTCCATCAGACAC | TCGCTGCCGCCTTTTCCTG | 158 |
| qPCR-FoxK1-γ | CAGAACTCCATCAGACACAACC | CAGGGGCGCTTCTGGAGG | 208 |
| qPCR-FoxK1-δ | CGTGACTCTGCCGGACTCTG | CTGACCAAGTAGCACAGCAAGATC | 174 |
| Primer pair . | Forward primer (5′→3′) . | Reverse primer (5′→3′) . | Amplicon size (bp) . |
|---|---|---|---|
| cDNA-FoxK1 | GGACGACACTGGAGCAAGA | GGGGCTCATTTTTGATCAAG | 1676 |
| ISH-FoxK1-α | GAGTCCAAGCCACCTTATTC | GGGGCTCATTTTTGATCAAG | 823 |
| ISH-FoxK1-γ | GAGTCCAAGCCACCTTATTC | CTGGAGCGCTCTGGGAGTAC | 703 |
| ISH-FoxK1-δ | GAGTCCAAGCCACCTTATTC | CTGGAGCGCTCTGGGAGTAC | 940 |
| qPCR-FoxK1-α | GCTGGCAGAACTCCATCAGACAC | TCGCTGCCGCCTTTTCCTG | 158 |
| qPCR-FoxK1-γ | CAGAACTCCATCAGACACAACC | CAGGGGCGCTTCTGGAGG | 208 |
| qPCR-FoxK1-δ | CGTGACTCTGCCGGACTCTG | CTGACCAAGTAGCACAGCAAGATC | 174 |
cDNA-FoxK1: sequences of the primers used to initially amplify the T. rubripes orthologue of FoxK1. ISH primer pairs: sequences used to subclone the three TFoxK1 splice variants for in situhybridisation. qPCR primer pairs: sequences used to quantify expression of the alternative TFoxK1 transcripts by real-time PCR