Primer and probe sequences for detection of glutamine synthetase(Onmy-GS01–GS04) and β-actin gene expression in rainbow trout Oncorhynchus mykiss using real time PCR
| Gene* . | Forward primer 5′–3′ . | Reverse primer 5′–3′ . | Probe 5′–3′ . | Length of amplicon (bp) . |
|---|---|---|---|---|
| Onmy-GS01 | CTGCAGTCTGTGTTCAGGGTAGA | CATCTGTCTGGAATTGTTAAGTCCATA | TACCTTTTGATCACTGCCAACATTGCCC | 101 |
| Onmy-GS02 | GGCAGTGTCTTTAAATGGCAACA | ACGCTACAATTGGCAAGACTGA | CTGTCTCCAGATTTGACACATTCCTGGATCAT | 136 |
| Onmy-GS03 | GTGTATCAATTTGCTACTCATGTTTAACAT | AAAATGGGTTCTTGATACAACTTCTACTAA | AAGGATCCAAGGTGCATCTGTGTTTTTATACATG | 191 |
| Onmy-GS04 | TTAATGAAAGATGGTGGCTGACA | CTGCAGGAAACGCGAGATC | CATTGTCTTCCCCTTTTGAGTCTTCTAGTGGG | 105 |
| β-actin | GACCCAGATCATGTTTGAGACCTT | CGTAGCCCTCGTAGATGGGTACT | ACTCCGGTGACGGCGTGACCC | 152 |
| Gene* . | Forward primer 5′–3′ . | Reverse primer 5′–3′ . | Probe 5′–3′ . | Length of amplicon (bp) . |
|---|---|---|---|---|
| Onmy-GS01 | CTGCAGTCTGTGTTCAGGGTAGA | CATCTGTCTGGAATTGTTAAGTCCATA | TACCTTTTGATCACTGCCAACATTGCCC | 101 |
| Onmy-GS02 | GGCAGTGTCTTTAAATGGCAACA | ACGCTACAATTGGCAAGACTGA | CTGTCTCCAGATTTGACACATTCCTGGATCAT | 136 |
| Onmy-GS03 | GTGTATCAATTTGCTACTCATGTTTAACAT | AAAATGGGTTCTTGATACAACTTCTACTAA | AAGGATCCAAGGTGCATCTGTGTTTTTATACATG | 191 |
| Onmy-GS04 | TTAATGAAAGATGGTGGCTGACA | CTGCAGGAAACGCGAGATC | CATTGTCTTCCCCTTTTGAGTCTTCTAGTGGG | 105 |
| β-actin | GACCCAGATCATGTTTGAGACCTT | CGTAGCCCTCGTAGATGGGTACT | ACTCCGGTGACGGCGTGACCC | 152 |
GenBank accession numbers: Onmy-GS01, AF390021; Onmy-GS02, AF390022; Onmy-GS03, AF390023; Onmy-GS04, AF390024; β-actin, AJ438158