Body composition of ad-libitum-fed mice and mice working for food
| . | . | . | . | Independent t-test . | . | |
|---|---|---|---|---|---|---|
| Variable (g) . | Ad-lib animals (N=7) . | Workload animals (N=15) . | Difference (%) . | t . | P . | |
| Body mass | 31.4±0.9 | 25.9±0.4 | -17 | -6.8 | <0.001 | |
| Dl mass | 8.1±0.2 | 6.6±0.1 | -19 | -9.9 | <0.001 | |
| Fat content | 3.13±0.35 | 0.94±0.09 | -70 | -8.2 | <0.001 | |
| Dl heart | 0.04±0.001 | 0.03±0.001 | -29 | -4.8 | <0.001 | |
| Dl liver | 0.48±0.03 | 0.30±0.02 | -37 | -5.0 | <0.001 | |
| Dl kidney | 0.13±0.01 | 0.10±0.001 | -29 | -4.0 | 0.001 | |
| Dl brain | 0.08±0.001 | 0.08±0.001 | -3 | -1.3 | 0.23 | |
| Dl stomach | 0.04±0.001 | 0.04±0.001 | +5 | 0.9 | 0.36 | |
| Dl intestines | 0.29±0.01 | 0.34±0.01 | +18 | 4.5 | <0.001 | |
| Dl lung | 0.04±0.001 | 0.04±0.001 | +3 | 0.6 | 0.59 | |
| Dl skin | 1.50±0.04 | 1.26±0.02 | -16 | -6.0 | <0.001 | |
| Dl rest | 5.48±0.12 | 4.35±0.05 | -21 | -10.6 | <0.001 | |
| Fat heart | 0.006±0.001 | 0.005±0.001 | -18 | -1.1 | 0.30 | |
| Fat liver | 0.048±0.009 | 0.027±0.003 | -43 | -2.8 | 0.011 | |
| Fat kidney | 0.026±0.003 | 0.006±0.001 | -75 | -7.7 | <0.001 | |
| Fat brain | 0.047±0.001 | 0.043±0.001 | -10 | -3.7 | 0.002 | |
| Fat stomach | 0.008±0.001 | 0.005±0.001 | -36 | -4.2 | <0.001 | |
| Fat intestines | 0.070±0.010 | 0.039±0.002 | -44 | -4.2 | <0.001 | |
| Fat lung | 0.008±0.001 | 0.004±0.001 | -51 | -6.7 | <0.001 | |
| Fat skin | 0.85±0.120 | 0.16±0.028 | -81 | -7.6 | <0.001 | |
| Fat rest | 2.07±0.240 | 0.65±0.071 | -69 | -7.4 | <0.001 | |
| . | . | . | . | Independent t-test . | . | |
|---|---|---|---|---|---|---|
| Variable (g) . | Ad-lib animals (N=7) . | Workload animals (N=15) . | Difference (%) . | t . | P . | |
| Body mass | 31.4±0.9 | 25.9±0.4 | -17 | -6.8 | <0.001 | |
| Dl mass | 8.1±0.2 | 6.6±0.1 | -19 | -9.9 | <0.001 | |
| Fat content | 3.13±0.35 | 0.94±0.09 | -70 | -8.2 | <0.001 | |
| Dl heart | 0.04±0.001 | 0.03±0.001 | -29 | -4.8 | <0.001 | |
| Dl liver | 0.48±0.03 | 0.30±0.02 | -37 | -5.0 | <0.001 | |
| Dl kidney | 0.13±0.01 | 0.10±0.001 | -29 | -4.0 | 0.001 | |
| Dl brain | 0.08±0.001 | 0.08±0.001 | -3 | -1.3 | 0.23 | |
| Dl stomach | 0.04±0.001 | 0.04±0.001 | +5 | 0.9 | 0.36 | |
| Dl intestines | 0.29±0.01 | 0.34±0.01 | +18 | 4.5 | <0.001 | |
| Dl lung | 0.04±0.001 | 0.04±0.001 | +3 | 0.6 | 0.59 | |
| Dl skin | 1.50±0.04 | 1.26±0.02 | -16 | -6.0 | <0.001 | |
| Dl rest | 5.48±0.12 | 4.35±0.05 | -21 | -10.6 | <0.001 | |
| Fat heart | 0.006±0.001 | 0.005±0.001 | -18 | -1.1 | 0.30 | |
| Fat liver | 0.048±0.009 | 0.027±0.003 | -43 | -2.8 | 0.011 | |
| Fat kidney | 0.026±0.003 | 0.006±0.001 | -75 | -7.7 | <0.001 | |
| Fat brain | 0.047±0.001 | 0.043±0.001 | -10 | -3.7 | 0.002 | |
| Fat stomach | 0.008±0.001 | 0.005±0.001 | -36 | -4.2 | <0.001 | |
| Fat intestines | 0.070±0.010 | 0.039±0.002 | -44 | -4.2 | <0.001 | |
| Fat lung | 0.008±0.001 | 0.004±0.001 | -51 | -6.7 | <0.001 | |
| Fat skin | 0.85±0.120 | 0.16±0.028 | -81 | -7.6 | <0.001 | |
| Fat rest | 2.07±0.240 | 0.65±0.071 | -69 | -7.4 | <0.001 | |
Values are mean ± s.e.m. Total dry lean (dl) mass, fat content and the dl mass and fat mass of separate organs are shown for Workload and Ad-lib mice. The body mass shown is the total body mass minus the gut and intestine content at time of death. One mouse died during the second respirometry measurement in the Workload group. Difference (%) shows the change in mass between Ad-lib and Workload animals. Independent t-tests were performed to test for differences between groups and the results are shown in the Table