Absolute amounts of free amino acids in the RBCs of Arctic char Salvelinus alpinus acclimated to freshwater or seawater for up to 96 h
| . | Free amino acid concentration (nmol g-1 wet mass tissue) . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Amino acid . | FW . | SW 24 h . | SW 48 h . | SW 96 h . | |||
| Essential | |||||||
| Histidine | 55.9±7.9 | 55.1±6.4 | 33.7±12.8 | 35.0±8.8 | |||
| Arginine | 81.3±6.8 | 97.1±8.6 | 70.2±11.0 | 96.0±12.8 | |||
| Threonine | 463.0±23.2 | 435.9±42.4 | 448.1±56.1 | 439.8±40.0 | |||
| Valine | 216.9±10.1 | 281.8±21.1 | 309.0±29.7* | 405.1±37.9*,† | |||
| Methionine | 141.0±10.6 | 134.8±18.8 | 142.7±21.9 | 151.6±20.5 | |||
| Tryptophan | 684.5±60.1 | 903.5±144.5 | 572.8±56.4 | 704.5±116.6 | |||
| Phenylalanine | 91.7±4.2 | 113.9±8.7 | 107.4±8.8 | 122.9±9.6* | |||
| Isoleucine | 134.8±5.5 | 175.3±17.4 | 220.6±18.7* | 283.4±20.3*,†,‡ | |||
| Leucine | 234.5±7.9 | 368.9±35.6* | 337.9±37.5* | 481.3±37.9*,‡ | |||
| Lysine | 108.1±7.2 | 130.0±24.0 | 104.1±29.6 | 106.2±15.3 | |||
| Total essential | 2211.8±75.7 | 2696.2±190.6* | 2346.4±144.7 | 2825.8±170.4* | |||
| Non-essential | |||||||
| Aspartate | 845.0±76.1 | 750.3±81.8 | 707.1±83.8 | 650.5±105.6 | |||
| Glutamate | 307.2±19.2 | 335.2±35.9 | 315.8±46.5 | 283.8±26.8 | |||
| Asparagine | 80.5±5.9 | 107.2±17.5 | 71.6±11.6 | 64.5±11.1 | |||
| Serine | 101.3±6.5 | 105.7±12.0 | 79.5±10.0 | 73.0±11.4 | |||
| Glutamine | 3157.5±145.9 | 3810.4±324.7 | 3479.9±408.5 | 3264.7±297.4 | |||
| Glycine | 993.9±99.2 | 904.6±108.5 | 1013.6±212.6 | 687.9±57.7 | |||
| Alanine | 415.9±27.7 | 544.4±70.4 | 455.1±85.5 | 452.4±56.7 | |||
| Taurine | 11616.9±488.3 | 13341.7±805.3 | 12301.3±798.0 | 13082.0±1024.9 | |||
| Tyrosine | 197.3±14.0 | 190.5±27.1 | 307.2±36.1*,† | 334.2±16.1*,† | |||
| Hydroxyproline | 901.5±94.2 | 794.5±139.1 | 737.2±91.9 | 827.8±194.7 | |||
| Proline | 87.9±25.9 | 82.9±16.1 | 50.6±10.8 | 57.5±18.5 | |||
| Total non-essential | 18704.6±559.6 | 20967.3±959.3 | 19519.0±1029.9 | 19778.3±1195.2 | |||
| Total FAA | 20916.4±571.1 | 23663.5±1087.5 | 21865.4±1081.8 | 22604.1±1279.0 | |||
| . | Free amino acid concentration (nmol g-1 wet mass tissue) . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Amino acid . | FW . | SW 24 h . | SW 48 h . | SW 96 h . | |||
| Essential | |||||||
| Histidine | 55.9±7.9 | 55.1±6.4 | 33.7±12.8 | 35.0±8.8 | |||
| Arginine | 81.3±6.8 | 97.1±8.6 | 70.2±11.0 | 96.0±12.8 | |||
| Threonine | 463.0±23.2 | 435.9±42.4 | 448.1±56.1 | 439.8±40.0 | |||
| Valine | 216.9±10.1 | 281.8±21.1 | 309.0±29.7* | 405.1±37.9*,† | |||
| Methionine | 141.0±10.6 | 134.8±18.8 | 142.7±21.9 | 151.6±20.5 | |||
| Tryptophan | 684.5±60.1 | 903.5±144.5 | 572.8±56.4 | 704.5±116.6 | |||
| Phenylalanine | 91.7±4.2 | 113.9±8.7 | 107.4±8.8 | 122.9±9.6* | |||
| Isoleucine | 134.8±5.5 | 175.3±17.4 | 220.6±18.7* | 283.4±20.3*,†,‡ | |||
| Leucine | 234.5±7.9 | 368.9±35.6* | 337.9±37.5* | 481.3±37.9*,‡ | |||
| Lysine | 108.1±7.2 | 130.0±24.0 | 104.1±29.6 | 106.2±15.3 | |||
| Total essential | 2211.8±75.7 | 2696.2±190.6* | 2346.4±144.7 | 2825.8±170.4* | |||
| Non-essential | |||||||
| Aspartate | 845.0±76.1 | 750.3±81.8 | 707.1±83.8 | 650.5±105.6 | |||
| Glutamate | 307.2±19.2 | 335.2±35.9 | 315.8±46.5 | 283.8±26.8 | |||
| Asparagine | 80.5±5.9 | 107.2±17.5 | 71.6±11.6 | 64.5±11.1 | |||
| Serine | 101.3±6.5 | 105.7±12.0 | 79.5±10.0 | 73.0±11.4 | |||
| Glutamine | 3157.5±145.9 | 3810.4±324.7 | 3479.9±408.5 | 3264.7±297.4 | |||
| Glycine | 993.9±99.2 | 904.6±108.5 | 1013.6±212.6 | 687.9±57.7 | |||
| Alanine | 415.9±27.7 | 544.4±70.4 | 455.1±85.5 | 452.4±56.7 | |||
| Taurine | 11616.9±488.3 | 13341.7±805.3 | 12301.3±798.0 | 13082.0±1024.9 | |||
| Tyrosine | 197.3±14.0 | 190.5±27.1 | 307.2±36.1*,† | 334.2±16.1*,† | |||
| Hydroxyproline | 901.5±94.2 | 794.5±139.1 | 737.2±91.9 | 827.8±194.7 | |||
| Proline | 87.9±25.9 | 82.9±16.1 | 50.6±10.8 | 57.5±18.5 | |||
| Total non-essential | 18704.6±559.6 | 20967.3±959.3 | 19519.0±1029.9 | 19778.3±1195.2 | |||
| Total FAA | 20916.4±571.1 | 23663.5±1087.5 | 21865.4±1081.8 | 22604.1±1279.0 | |||
Values are means ± s.e.m.; N=8 for SW 24, 48 and 96 h groups, N=24 for the pooled FW group
Free amino acid (FAA) levels for FW char have been pooled since there were no significant differences between the 24, 48 and 96 h sample groups. SW,seawater, 32‰
Significantly different from pooled freshwater (FW; control) value
significantly different from SW 24 h group
significantly different from SW 48 h group