Absolute amounts of free amino acids in the red muscle of Arctic char Salvelinus alpinus acclimated to freshwater or seawater for up to 96 h
| . | Free amino acid concentration (nmol g-1 wet mass tissue) . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Amino acid . | FW . | SW 24 h . | SW 48 h . | SW 96 h . | |||
| Essential | |||||||
| Histidine | 178.8±24.0 | 218.7±51.9 | 255.4±38.0 | 211.4±50.2 | |||
| Arginine | 12.8±3.3 | 12.7±2.7 | 19.2±2.5 | 25.6±2.9 | |||
| Threonine | 227.7±18.0 | 247.9±25.3 | 312.2±17.9 | 282.1±39.1 | |||
| Valine | 90.6±6.9 | 96.3±13.7 | 123.6±11.0 | 149.2±9.8*,† | |||
| Methionine | 93.7±5.8 | 100.1±14.0 | 108.2±8.1 | 107.1±6.9 | |||
| Tryptophan | 483.0±26.3 | 470.2±31.2 | 528.0±47.2 | 535.6±92.4 | |||
| Phenylalanine | 12.6±2.9 | 16.2±4.9 | 24.7±3.0 | 30.4±4.7*,† | |||
| Isoleucine | 37.4±3.8 | 43.0±6.6 | 64.9±8.1*,† | 89.4±5.6*,†,‡ | |||
| Leucine | 72.9±5.5 | 83.3±10.1 | 117.3±14.1*,† | 147.4±6.2*,†,‡ | |||
| Lysine | 76.4±12.7 | 67.6±10.8 | 90.1±16.1 | 85.6±11.8 | |||
| Total essential | 1285.9±90.9 | 1356.2±147.0 | 1643.7±105.7 | 1663.7±210.5 | |||
| Non-essential | |||||||
| Aspartate | 426.8±43.2 | 415.4±124.3 | 414.2±68.9 | 413.6±91.4 | |||
| Glutamate | 520.2±32.3 | 576.4±72.1 | 540.7±42.7 | 587.7±51.6 | |||
| Asparagine | 197.7±21.6 | 337.2±140.0 | 324.7±45.3 | 236.4±61.1 | |||
| Serine | 78.6±10.3 | 139.7±39.2 | 135.5±21.7 | 100.9±11.8 | |||
| Glutamine | 194.2±13.1 | 268.7±44.7 | 303.5±22.3* | 241.5±34.5 | |||
| Glycine | 444.5±103.5 | 525.7±170.1 | 471.8±96.9 | 408.7±80.5 | |||
| Alanine | 723.1±46.3 | 912.3±141.2 | 978.8±132.5 | 829.4±102.1 | |||
| Taurine | 12814.2±449.1 | 12199.9±913.0 | 12681.2±565.4 | 13303.1±1407.8 | |||
| Tyrosine | 70.7±5.9 | 83.1±9.7 | 79.7±15.3 | 81.2±11.3 | |||
| Hydroxyproline | 1372.4±200.4 | 2202.3±543.9 | 2069.8±635.5 | 2869.3±1396.8 | |||
| Proline | 430.5±133.1 | 823.2±441.6 | 332.8±155.5 | 647.9±318.5 | |||
| Total non-essential | 17272.9±532.6 | 18483.9±1772.5 | 18332.8±1220.4 | 19746.6±1649.2 | |||
| Total FAA | 18558.8±582.3 | 19840.1±1914.2 | 19976.5±1302.0 | 21410.3±1809.6 | |||
| . | Free amino acid concentration (nmol g-1 wet mass tissue) . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Amino acid . | FW . | SW 24 h . | SW 48 h . | SW 96 h . | |||
| Essential | |||||||
| Histidine | 178.8±24.0 | 218.7±51.9 | 255.4±38.0 | 211.4±50.2 | |||
| Arginine | 12.8±3.3 | 12.7±2.7 | 19.2±2.5 | 25.6±2.9 | |||
| Threonine | 227.7±18.0 | 247.9±25.3 | 312.2±17.9 | 282.1±39.1 | |||
| Valine | 90.6±6.9 | 96.3±13.7 | 123.6±11.0 | 149.2±9.8*,† | |||
| Methionine | 93.7±5.8 | 100.1±14.0 | 108.2±8.1 | 107.1±6.9 | |||
| Tryptophan | 483.0±26.3 | 470.2±31.2 | 528.0±47.2 | 535.6±92.4 | |||
| Phenylalanine | 12.6±2.9 | 16.2±4.9 | 24.7±3.0 | 30.4±4.7*,† | |||
| Isoleucine | 37.4±3.8 | 43.0±6.6 | 64.9±8.1*,† | 89.4±5.6*,†,‡ | |||
| Leucine | 72.9±5.5 | 83.3±10.1 | 117.3±14.1*,† | 147.4±6.2*,†,‡ | |||
| Lysine | 76.4±12.7 | 67.6±10.8 | 90.1±16.1 | 85.6±11.8 | |||
| Total essential | 1285.9±90.9 | 1356.2±147.0 | 1643.7±105.7 | 1663.7±210.5 | |||
| Non-essential | |||||||
| Aspartate | 426.8±43.2 | 415.4±124.3 | 414.2±68.9 | 413.6±91.4 | |||
| Glutamate | 520.2±32.3 | 576.4±72.1 | 540.7±42.7 | 587.7±51.6 | |||
| Asparagine | 197.7±21.6 | 337.2±140.0 | 324.7±45.3 | 236.4±61.1 | |||
| Serine | 78.6±10.3 | 139.7±39.2 | 135.5±21.7 | 100.9±11.8 | |||
| Glutamine | 194.2±13.1 | 268.7±44.7 | 303.5±22.3* | 241.5±34.5 | |||
| Glycine | 444.5±103.5 | 525.7±170.1 | 471.8±96.9 | 408.7±80.5 | |||
| Alanine | 723.1±46.3 | 912.3±141.2 | 978.8±132.5 | 829.4±102.1 | |||
| Taurine | 12814.2±449.1 | 12199.9±913.0 | 12681.2±565.4 | 13303.1±1407.8 | |||
| Tyrosine | 70.7±5.9 | 83.1±9.7 | 79.7±15.3 | 81.2±11.3 | |||
| Hydroxyproline | 1372.4±200.4 | 2202.3±543.9 | 2069.8±635.5 | 2869.3±1396.8 | |||
| Proline | 430.5±133.1 | 823.2±441.6 | 332.8±155.5 | 647.9±318.5 | |||
| Total non-essential | 17272.9±532.6 | 18483.9±1772.5 | 18332.8±1220.4 | 19746.6±1649.2 | |||
| Total FAA | 18558.8±582.3 | 19840.1±1914.2 | 19976.5±1302.0 | 21410.3±1809.6 | |||
Values are means ± s.e.m.; N=8 for SW 24, 48 and 96 h groups, N=24 for the pooled FW group
Free amino acid (FAA) levels for FW char have been pooled since there were no significant differences between the 24, 48 and 96 h sample groups. SW,seawater, 32‰
Significantly different from pooled freshwater (FW; control) value
significantly different from SW 24 h group
significantly different from SW 48 h group