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Table 1.

HPLC gradient protocol for carbohydrate probe derivative separation*

Time (min)Solvent A (%)Solvent B (%)
0 (initial conditions) 90 10 
5.5 90 10 
80 20 
12 66 34 
13 (wash) 100 
16 100 
18 (equilibrate) 90 10 
23 (end time) 90 10 
Time (min)Solvent A (%)Solvent B (%)
0 (initial conditions) 90 10 
5.5 90 10 
80 20 
12 66 34 
13 (wash) 100 
16 100 
18 (equilibrate) 90 10 
23 (end time) 90 10 
*

See Materials and methods for solvent composition, flow rate and column type. All changes in proportional solvent composition were linear over time.

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