. | . | Target (surface charge) . | . | . | . | |||
---|---|---|---|---|---|---|---|---|
Sample . | Haemocyte viability . | DEAE (+) . | DEAE+heparin (-) . | CM (-) . | CM+poly-l-lysine (+) . | |||
With plasma | Viable | +++ | ++a | ++a | +++ | |||
Without plasma | Viable | ++b | 0 | 0 | ++b | |||
With plasma | Non-viable | + | 0 | 0 | + | |||
Without plasma | Non-viable | ++b | 0 | 0 | ++b |
. | . | Target (surface charge) . | . | . | . | |||
---|---|---|---|---|---|---|---|---|
Sample . | Haemocyte viability . | DEAE (+) . | DEAE+heparin (-) . | CM (-) . | CM+poly-l-lysine (+) . | |||
With plasma | Viable | +++ | ++a | ++a | +++ | |||
Without plasma | Viable | ++b | 0 | 0 | ++b | |||
With plasma | Non-viable | + | 0 | 0 | + | |||
Without plasma | Non-viable | ++b | 0 | 0 | ++b |
+++, very strong encapsulation response, characterized by >90%haemocytes in well attached to beads, formation of large, tightly-bound bead/haemocyte aggregates (containing >150 beads) with haemocytes flattened and spread on the bead surface.
++, less strong encapsulation response, characterized by ca. 75% haemocytes in well attached to beads, formation of smaller bead/haemocyte aggregates(containing <10 beads). aTightly bound bead/haemocyte aggregates, haemocytes flattened and spread, bloosely bound bead/haemocyte aggregates, haemocytes remain rounded after attachment.
+, weak encapsulation response, characterized by <30% haemocytes in well attached to beads, formation of very large, loosely bound bead/haemocyte aggregates (containing >250 beads), held together with few rounded haemocytes.
0, no encapsulation.
N=10 for each preparation.