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Table 2.

Gene expression after freshwater transfer in killifish intestine

Time after transfer
GeneSalinity12 h3 days7 days
Na+/K+-ATPase α1a BW 1.00±0.23 0.74±0.12 0.77±0.07 
 FW 0.95±0.13 0.82±0.13 0.74±0.07 
NKCC2 BW 1.00±0.25 0.72±0.18 1.26±0.26 
 FW 0.91±0.15 0.73±0.16 0.59±0.13 
CFTR BW 1.00±0.10 0.78±0.07 0.77±0.06 
 FW 0.99±0.10 0.95±0.10 0.95±0.06 
CA2 BW 1.00±0.07 0.68±0.07* 0.69±0.03* 
 FW 0.90±0.10 0.60±0.06* 0.58±0.03* 
14-3-3a BW 1.00±0.19 0.82±0.07 0.56±0.03* 
 FW 1.24±0.15 0.68±0.07 0.67±0.06 
Time after transfer
GeneSalinity12 h3 days7 days
Na+/K+-ATPase α1a BW 1.00±0.23 0.74±0.12 0.77±0.07 
 FW 0.95±0.13 0.82±0.13 0.74±0.07 
NKCC2 BW 1.00±0.25 0.72±0.18 1.26±0.26 
 FW 0.91±0.15 0.73±0.16 0.59±0.13 
CFTR BW 1.00±0.10 0.78±0.07 0.77±0.06 
 FW 0.99±0.10 0.95±0.10 0.95±0.06 
CA2 BW 1.00±0.07 0.68±0.07* 0.69±0.03* 
 FW 0.90±0.10 0.60±0.06* 0.58±0.03* 
14-3-3a BW 1.00±0.19 0.82±0.07 0.56±0.03* 
 FW 1.24±0.15 0.68±0.07 0.67±0.06 

Values are means ± s.e.m. (N≥7).

BW, brackish water control; FW, fresh water; NKCC2,Na+/K+/2Cl- cotransporters; CFTR, cystic fibrosis transmembrane conductance regulator; CA2, carbonic anhydrase 2;14-3-3a, signalling protein 14-3-3a.

*

Significant difference from 12 h BW group (P<0.05). There were no significant differences between FW and time-matched BW groups. qRT-PCR expression data are normalized to 12 h brackish water controls.

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