Gene expression after freshwater transfer in killifish intestine
| . | . | Time after transfer . | . | . | ||
|---|---|---|---|---|---|---|
| Gene . | Salinity . | 12 h . | 3 days . | 7 days . | ||
| Na+/K+-ATPase α1a | BW | 1.00±0.23 | 0.74±0.12 | 0.77±0.07 | ||
| FW | 0.95±0.13 | 0.82±0.13 | 0.74±0.07 | |||
| NKCC2 | BW | 1.00±0.25 | 0.72±0.18 | 1.26±0.26 | ||
| FW | 0.91±0.15 | 0.73±0.16 | 0.59±0.13 | |||
| CFTR | BW | 1.00±0.10 | 0.78±0.07 | 0.77±0.06 | ||
| FW | 0.99±0.10 | 0.95±0.10 | 0.95±0.06 | |||
| CA2 | BW | 1.00±0.07 | 0.68±0.07* | 0.69±0.03* | ||
| FW | 0.90±0.10 | 0.60±0.06* | 0.58±0.03* | |||
| 14-3-3a | BW | 1.00±0.19 | 0.82±0.07 | 0.56±0.03* | ||
| FW | 1.24±0.15 | 0.68±0.07 | 0.67±0.06 | |||
| . | . | Time after transfer . | . | . | ||
|---|---|---|---|---|---|---|
| Gene . | Salinity . | 12 h . | 3 days . | 7 days . | ||
| Na+/K+-ATPase α1a | BW | 1.00±0.23 | 0.74±0.12 | 0.77±0.07 | ||
| FW | 0.95±0.13 | 0.82±0.13 | 0.74±0.07 | |||
| NKCC2 | BW | 1.00±0.25 | 0.72±0.18 | 1.26±0.26 | ||
| FW | 0.91±0.15 | 0.73±0.16 | 0.59±0.13 | |||
| CFTR | BW | 1.00±0.10 | 0.78±0.07 | 0.77±0.06 | ||
| FW | 0.99±0.10 | 0.95±0.10 | 0.95±0.06 | |||
| CA2 | BW | 1.00±0.07 | 0.68±0.07* | 0.69±0.03* | ||
| FW | 0.90±0.10 | 0.60±0.06* | 0.58±0.03* | |||
| 14-3-3a | BW | 1.00±0.19 | 0.82±0.07 | 0.56±0.03* | ||
| FW | 1.24±0.15 | 0.68±0.07 | 0.67±0.06 | |||
Values are means ± s.e.m. (N≥7).
BW, brackish water control; FW, fresh water; NKCC2,Na+/K+/2Cl- cotransporters; CFTR, cystic fibrosis transmembrane conductance regulator; CA2, carbonic anhydrase 2;14-3-3a, signalling protein 14-3-3a.
Significant difference from 12 h BW group (P<0.05). There were no significant differences between FW and time-matched BW groups. qRT-PCR expression data are normalized to 12 h brackish water controls.