The Company of Biologists

Table 1.

Activities of enzymes of intermediary metabolism in liver, kidney and heart from the freshwater ocellate river stingray (Potamotrygon motoro),white-edge whip ray (Himantura signifer) in freshwater, H. signifer in half-strength seawater, the marine blue-spotted ribbontail stingray (Taeniura lymma), and the seawater brownbanded bamboo shark (Chiloscyllium punctatum)

	P. motoro (FW)	H. signifer (FW)	H. signifer (½ SW)	T. lymma (SW)	C. punctatum (SW)
Liver
CCO	3.21±0.42^e,KH	4.32±0.28 (8)^e,KH	4.81±0.45 (7)^e,KH	5.54±0.86^e	17.0±1.6 (4)^abcd,H
CPT	0.27±0.03^bcde,KH	0.77±0.07 (9)^a,KH	0.63±0.05 (7)^a,KH	0.62±0.04^a,KH	1.09±0.28 (3)^a,H
COT	0.26±0.04^bc,KH	1.26±0.26 (9)^ad,KH	0.71±0.14 (7)^ad,KH	0.18±0.04^bce,KH	0.64±0.13 (3)^d,H
HOAD	2.55±0.43^bc,KH	6.90±0.57 (9)^ade,KH	7.40±1.01 (7)^ade,KH	4.98±0.35^bc,KH	2.53±0.13 (4)^bc,KH
d-β-HBDH	6.03±0.99^e,K	8.47±0.66^de,KH	7.81±0.93^de,K	3.77±0.39^bc,KH	2.08±0.37(4)^abc,H
PK	16.3±0.7^de,KH	15.5±1.2^de,KH	14.9±0.7^de,KH	21.9±1.1^abce,KH	7.31±1.05 (4)^abcd,KH
GDH	36.0±5.4^de,KH	31.7±3.4 (9)^de,KH	25.7±2.6 (7)^de,KH	6.33±0.45^abc,K	9.62±0.40 (4)^abc,KH
Kidney
CCO	9.53±0.97^e,L	14.2±1.6^e,L	15.4±0.85^L	10.2±1.6 (5)^e	21.7±2.3^abd,H
CPT	0.13±0.01^de,LH	0.17±0.02^de,LH	0.15±0.01^de,L	1.31±0.06^abce,LH	0.53±0.04^abcd,H
COT	0.082±0.021^bcde,L	0.20±0.01^ade,L	0.20±0.01^ade,L	0.29±0.04 ^abce,LH	0.53±0.07^abcd,H
HOAD	0.55±0.04^de,L	0.82±0.09^d,L	0.83±0.04^d,L	1.71±0.17^abce,LH	0.98±0.10^ad,L
d-β-HBDH	2.28±0.28^de,L	2.21±0.12^de,LH	1.90±0.09^de,LH	0.04±0.02^abce,LH	0.92±0.12^abcd
PK	81.1±4.3^bcde,L	54.9±1.3^ae,LH	55.6±1.9^ae,LH	58.4±1.4^ae,LH	27.0±2.7^abcd,LH
GDH	13.7±1.4^de,LK	9.62±1.04^de,L	12.6±0.8^de,LH,*	53.4±3.3^abce,LH	26.5±2.7^abcd,LH
Heart
CCO	12.3±0.7 (4)^de,L	12.9±2.0 (5)^de,L	14.4±1.4 (5)^de,L	5.87±0.95 (4)^abce	39.9±2.7^abcd,LK
CPT	0.033±0.010^bcd,LK	0.072±0.003^ae,LK	0.088±0.014^ae,L	0.085±0.008^ae,LK	0.010±0.004^bcd,LK
COT	0.060±0.017^bc,L,†	0.199±0.016^ade,L,†	0.182±0.016^ade,L,†	0.060±0.019^bc,LK	0.066±0.019^bc,LK,†
HOAD	0.37±0.03 (5)^bcde,L	0.72±0.07^ae,L	0.70±0.08^ae,L	0.78±0.09^ae,LK	1.23±0.10^abcd,L
d-β-HBDH	3.82±0.20 (5)^bce	10.9±0.6^ade,LK	9.50±0.90^ade,K	2.48±0.43^bc,LK	0.87±0.24^abc,L
PK	83.9±5.7 (5)^L	77.5±4.1^LK	85.0±7.4^LK	104.1±4.7^LK	84.0±16.4^LK
GDH	2.66±0.21 (5)^b,LH	4.60±0.32^a,L	4.19±0.30^LK	3.31±0.67^K	4.24±0.30^LK

	P. motoro (FW)	H. signifer (FW)	H. signifer (½ SW)	T. lymma (SW)	C. punctatum (SW)
Liver
CCO	3.21±0.42^e,KH	4.32±0.28 (8)^e,KH	4.81±0.45 (7)^e,KH	5.54±0.86^e	17.0±1.6 (4)^abcd,H
CPT	0.27±0.03^bcde,KH	0.77±0.07 (9)^a,KH	0.63±0.05 (7)^a,KH	0.62±0.04^a,KH	1.09±0.28 (3)^a,H
COT	0.26±0.04^bc,KH	1.26±0.26 (9)^ad,KH	0.71±0.14 (7)^ad,KH	0.18±0.04^bce,KH	0.64±0.13 (3)^d,H
HOAD	2.55±0.43^bc,KH	6.90±0.57 (9)^ade,KH	7.40±1.01 (7)^ade,KH	4.98±0.35^bc,KH	2.53±0.13 (4)^bc,KH
d-β-HBDH	6.03±0.99^e,K	8.47±0.66^de,KH	7.81±0.93^de,K	3.77±0.39^bc,KH	2.08±0.37(4)^abc,H
PK	16.3±0.7^de,KH	15.5±1.2^de,KH	14.9±0.7^de,KH	21.9±1.1^abce,KH	7.31±1.05 (4)^abcd,KH
GDH	36.0±5.4^de,KH	31.7±3.4 (9)^de,KH	25.7±2.6 (7)^de,KH	6.33±0.45^abc,K	9.62±0.40 (4)^abc,KH
Kidney
CCO	9.53±0.97^e,L	14.2±1.6^e,L	15.4±0.85^L	10.2±1.6 (5)^e	21.7±2.3^abd,H
CPT	0.13±0.01^de,LH	0.17±0.02^de,LH	0.15±0.01^de,L	1.31±0.06^abce,LH	0.53±0.04^abcd,H
COT	0.082±0.021^bcde,L	0.20±0.01^ade,L	0.20±0.01^ade,L	0.29±0.04 ^abce,LH	0.53±0.07^abcd,H
HOAD	0.55±0.04^de,L	0.82±0.09^d,L	0.83±0.04^d,L	1.71±0.17^abce,LH	0.98±0.10^ad,L
d-β-HBDH	2.28±0.28^de,L	2.21±0.12^de,LH	1.90±0.09^de,LH	0.04±0.02^abce,LH	0.92±0.12^abcd
PK	81.1±4.3^bcde,L	54.9±1.3^ae,LH	55.6±1.9^ae,LH	58.4±1.4^ae,LH	27.0±2.7^abcd,LH
GDH	13.7±1.4^de,LK	9.62±1.04^de,L	12.6±0.8^de,LH,*	53.4±3.3^abce,LH	26.5±2.7^abcd,LH
Heart
CCO	12.3±0.7 (4)^de,L	12.9±2.0 (5)^de,L	14.4±1.4 (5)^de,L	5.87±0.95 (4)^abce	39.9±2.7^abcd,LK
CPT	0.033±0.010^bcd,LK	0.072±0.003^ae,LK	0.088±0.014^ae,L	0.085±0.008^ae,LK	0.010±0.004^bcd,LK
COT	0.060±0.017^bc,L,†	0.199±0.016^ade,L,†	0.182±0.016^ade,L,†	0.060±0.019^bc,LK	0.066±0.019^bc,LK,†
HOAD	0.37±0.03 (5)^bcde,L	0.72±0.07^ae,L	0.70±0.08^ae,L	0.78±0.09^ae,LK	1.23±0.10^abcd,L
d-β-HBDH	3.82±0.20 (5)^bce	10.9±0.6^ade,LK	9.50±0.90^ade,K	2.48±0.43^bc,LK	0.87±0.24^abc,L
PK	83.9±5.7 (5)^L	77.5±4.1^LK	85.0±7.4^LK	104.1±4.7^LK	84.0±16.4^LK
GDH	2.66±0.21 (5)^b,LH	4.60±0.32^a,L	4.19±0.30^LK	3.31±0.67^K	4.24±0.30^LK

FW, freshwater; SW, sea water; ½ SW, half-strength (15‰)seawater.

Values are mean ± s.e.m.; N=6 except where noted in parentheses.

Activities were measured at 25°C. ND, not detectable. See List of abbreviations for enzyme names.

Activities are expressed as μmol min^-1 g^-1 wet mass. Lowercase letters in superscript indicate a significant difference(one-way ANOVA, P<0.05) from the same enzyme in the same tissue: ^aP. motoro, ^bH. signifer (FW), ^cH. signifer (½ SW), ^dT. lymma, ^eC. punctatum. Uppercase letters in superscript indicate a significant difference (one-way ANOVA, P<0.05) from the same enzyme activity in the same species in: ^Lliver, ^Kkidney, ^Hheart; *significant difference between H. signifer (FW)and H. signifer (1/2SW) (Student's t-test, P<0.05); ^†COT activity is significantly different from CPT activity within the same tissue (Student's t-test, P<0.05). ND activities were omitted from statistical tests.

View Large

This Feature Is Available To Subscribers Only