Primer sequences and the lengths of the amplified fragments
| Amplified fragment . | Length (bp) . | Location . | Primer sequence . |
|---|---|---|---|
| D-loop | 1100 | L strand (15294-15320) | 5′-ATAAACATTACTCTGGTCTTGTAAACC-3′ |
| H strand (98-72) | 5′-ATTAATAAGGCCAGGACCAAACCT-3′ | ||
| tRNAMet+Glu+Ile | 1126 | L strand (3401-3419) | 5′-CGGCCCATTCGCGTTATTC-3′ |
| H strand (4527-4508) | 5′-AGGTTGAGTAGAGTGAGGGA-3′ | ||
| ND3 fragment | 534 | L strand (9364-9385) | 5′-ACGTCTCCATTTATTGATGAGG-3′ |
| H strand (9897-9876) | 5′-GAGGTTGAAGAAGGTAGATGGC-3′ | ||
| D-loop 5′ fragment | 342 | L strand (15371-15389) | 5′-CCACCACCAGCACCCAAAG-3′ |
| H strand (15712-15694) | 5′-CGGGTTGTTGGTTTCACGG-3′ | ||
| D-loop 3′ fragment | 437 | L strand (15950-15968) | 5′-AGGCATGAAAGGACAGCAC-3′ |
| H strand (91-73) | 5′-ATAAGGCCAGGACCAAACC-3′ |
| Amplified fragment . | Length (bp) . | Location . | Primer sequence . |
|---|---|---|---|
| D-loop | 1100 | L strand (15294-15320) | 5′-ATAAACATTACTCTGGTCTTGTAAACC-3′ |
| H strand (98-72) | 5′-ATTAATAAGGCCAGGACCAAACCT-3′ | ||
| tRNAMet+Glu+Ile | 1126 | L strand (3401-3419) | 5′-CGGCCCATTCGCGTTATTC-3′ |
| H strand (4527-4508) | 5′-AGGTTGAGTAGAGTGAGGGA-3′ | ||
| ND3 fragment | 534 | L strand (9364-9385) | 5′-ACGTCTCCATTTATTGATGAGG-3′ |
| H strand (9897-9876) | 5′-GAGGTTGAAGAAGGTAGATGGC-3′ | ||
| D-loop 5′ fragment | 342 | L strand (15371-15389) | 5′-CCACCACCAGCACCCAAAG-3′ |
| H strand (15712-15694) | 5′-CGGGTTGTTGGTTTCACGG-3′ | ||
| D-loop 3′ fragment | 437 | L strand (15950-15968) | 5′-AGGCATGAAAGGACAGCAC-3′ |
| H strand (91-73) | 5′-ATAAGGCCAGGACCAAACC-3′ |
L strand, light strand of mtDNA; H strand, heavy strand of mtDNA.
PCR reactions were done in a volume of 100 μl and included 0.5 μg mtDNA as template. Amplification in the PCR consisted of an initial denaturation step at 94°C for 5 min followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 1 min, and extension at 72°C for 1 min. Following the final cycle, the mixture was incubated at 72°C for 10 min.