CA activity and iodide inhibition in wild-type and CAII-deficient mice
| . | Genotype . | Specific CA activity (U ml mg-1 protein) . | Inhibition with 7 mmol l-1 KI (%) . |
|---|---|---|---|
| Red cell lysate | Wild type | 71 100±10 600 (N=7) | 44±4 (N=24) |
| CAII-deficient | 887±132 (N=6) | 100±0 (N=9) | |
| Cytosol caecum | Wild type | 289±45 (N=7) | 65±4 (N=7) |
| CAII-deficient | 96±34 (N=6) | 100±0 (N=6) | |
| Cytosol proximal colon | Wild type | 186±35 (N=7) | 61±6 (N=7) |
| CAII-deficient | 58±19 (N=6) | 100±0 (N=6) | |
| Cytosol distal colon | Wild type | 195±36 (N=7) | 54±6 (N=7) |
| CAII-deficient | 5±2 (N=6) | 100±0 (N=6) | |
| Mucus caecum | Wild type | 30±12 (N=7) | 50±7 (N=7) |
| CAII-deficient | 42±9 (N=6) | 55±11 (N=6) | |
| Mucus proximal colon | Wild type | 20±9 (N=7) | 35±10 (N=7) |
| CAII-deficient | 40±15 (N=6) | 56±9 (N=5) | |
| Mucus distal colon | Wild type | 10±9 (N=7) | — |
| CAII-deficient | 21±16 (N=6) | 33±9 (N=6) | |
| Saliva | Wild type | 63±25 (N=5) | 46±13 (N=5) |
| CAII-deficient | 83±39 (N=5) | 41±10 (N=5) |
| . | Genotype . | Specific CA activity (U ml mg-1 protein) . | Inhibition with 7 mmol l-1 KI (%) . |
|---|---|---|---|
| Red cell lysate | Wild type | 71 100±10 600 (N=7) | 44±4 (N=24) |
| CAII-deficient | 887±132 (N=6) | 100±0 (N=9) | |
| Cytosol caecum | Wild type | 289±45 (N=7) | 65±4 (N=7) |
| CAII-deficient | 96±34 (N=6) | 100±0 (N=6) | |
| Cytosol proximal colon | Wild type | 186±35 (N=7) | 61±6 (N=7) |
| CAII-deficient | 58±19 (N=6) | 100±0 (N=6) | |
| Cytosol distal colon | Wild type | 195±36 (N=7) | 54±6 (N=7) |
| CAII-deficient | 5±2 (N=6) | 100±0 (N=6) | |
| Mucus caecum | Wild type | 30±12 (N=7) | 50±7 (N=7) |
| CAII-deficient | 42±9 (N=6) | 55±11 (N=6) | |
| Mucus proximal colon | Wild type | 20±9 (N=7) | 35±10 (N=7) |
| CAII-deficient | 40±15 (N=6) | 56±9 (N=5) | |
| Mucus distal colon | Wild type | 10±9 (N=7) | — |
| CAII-deficient | 21±16 (N=6) | 33±9 (N=6) | |
| Saliva | Wild type | 63±25 (N=5) | 46±13 (N=5) |
| CAII-deficient | 83±39 (N=5) | 41±10 (N=5) |
Values are means ± s.d.