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Table 3.

Indices of metabolic activity in Rana sylvatica organs incubated in PBS or PBS containing 80 mmol l−1 urea

No ureaUreaP
Heart 3.6±0.5 3.8±1.4 0.593 
Liver 4.9±0.4 3.6±0.3 0.002 
Kidney 4.1±0.2 4.0±0.3 0.593 
Gastrocnemius 5.7±0.4 4.9±0.4 0.021 
No ureaUreaP
Heart 3.6±0.5 3.8±1.4 0.593 
Liver 4.9±0.4 3.6±0.3 0.002 
Kidney 4.1±0.2 4.0±0.3 0.593 
Gastrocnemius 5.7±0.4 4.9±0.4 0.021 

Values are means ± S.E.M.; N=6 (heart, kidney) or N=12 (liver, gastrocnemius) replicates per group.

Metabolic index is the percent reduction of indicator dye per milligram dry tissue per hour at 15°C.

Within each row, means were compared using paired Student's t-test. Statistical significance is taken at the level of P<0.05.

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