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Table 1.

Concentrations of urea and glucose (μmol g−1dry tissue) in organs of unfrozen and frozen Rana sylvatica

UreaGlucoseP
Unfrozen    
Liver 48.1±5.2 26.7±5.1 0.049 
Gut 63.8±4.4 9.0±1.7 <0.001 
Flexor carpi 56.5±3.8 6.5±1.4 <0.001 
Gracilis 47.6±3.8 6.6±1.8 <0.001 
Frozen    
Liver 56.5±3.2 946.0±82.6* <0.001 
Gut 66.9±6.1 174.0±20.6* 0.004 
Flexor carpi 45.2±8.0 49.5±5.7* 0.453 
Gracilis 28.1±5.2* 51.9±10.2* 0.006 
UreaGlucoseP
Unfrozen    
Liver 48.1±5.2 26.7±5.1 0.049 
Gut 63.8±4.4 9.0±1.7 <0.001 
Flexor carpi 56.5±3.8 6.5±1.4 <0.001 
Gracilis 47.6±3.8 6.6±1.8 <0.001 
Frozen    
Liver 56.5±3.2 946.0±82.6* <0.001 
Gut 66.9±6.1 174.0±20.6* 0.004 
Flexor carpi 45.2±8.0 49.5±5.7* 0.453 
Gracilis 28.1±5.2* 51.9±10.2* 0.006 

Values are means ± s.e.m.; N=5 replicates per group.

Means within each row were compared using dependent-measures Student's t-tests; P as indicated. Asterisk denotes that the value differed significantly (unpaired Student's t-test; P<0.05) from the corresponding mean for unfrozen frogs.

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