Primer sequences used to amplify OAT1-like, OAT3-like, MRP2-like and MRP4-like cDNA from PTCs
| Transporter . | . | Primer sequence . |
|---|---|---|
| OAT1 | Forward | 5′-TGGTTCTCCACCAGCTTTGC-3′ |
| Reverse | 5′-TTCAGGGAGGAAAAGAGCAGCG-3′ | |
| OAT3 | Forward | 5′-CCCTTCTTCCTCTTCTTCCTCG-3′ |
| Reverse | 5′-TGGATCAGATAAATGCTGACCCC-3′ | |
| MRP2 | Forward | 5′-AAATCCTCCCTCACCAACTGCC-3′ |
| Reverse | 5′-TTCGCCTTGCAGAGAAGACG-3′ | |
| MRP4 | Forward | 5′-CGAGGCAGTGGTTAGCATACGAC-3′ |
| Reverse | 5′-CGCACTCAGTGGATCGTCCAA-3′ |
| Transporter . | . | Primer sequence . |
|---|---|---|
| OAT1 | Forward | 5′-TGGTTCTCCACCAGCTTTGC-3′ |
| Reverse | 5′-TTCAGGGAGGAAAAGAGCAGCG-3′ | |
| OAT3 | Forward | 5′-CCCTTCTTCCTCTTCTTCCTCG-3′ |
| Reverse | 5′-TGGATCAGATAAATGCTGACCCC-3′ | |
| MRP2 | Forward | 5′-AAATCCTCCCTCACCAACTGCC-3′ |
| Reverse | 5′-TTCGCCTTGCAGAGAAGACG-3′ | |
| MRP4 | Forward | 5′-CGAGGCAGTGGTTAGCATACGAC-3′ |
| Reverse | 5′-CGCACTCAGTGGATCGTCCAA-3′ |
The primers were generated against known sequences for OAT1-like (BBSRC Chick EST ID 603807902F1), OAT3-like (BBSRC Chick EST ID 603812145F1),MRP2-like (GenBank accession no. XM_421698) and MRP4-like (GenBank accession no. XM_416986) transporters from the domestic chicken (Gallus gallus).