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Table 1.

Haemolymph respiratory and acid–base status of laboratory acclimated Discoplax hirtipes at rest or after 5 min of exercise at either 40% or 90% relative humidity (RH)

	P_O₂ (kPa)					[O₂] (mmol l^-1)
	Pulmonary	Arterial	Venous	Pulmonary	Arterial	Venous	Pulmonary	Arterial	Venous
Resting	12.75±0.64	8.88±1.67^p	1.05±0.12^a,p	0.99±0.12	0.87±0.12	0.46±0.07^a	96.65±6.54	83.58±4.61	44.80±4.21^a,p
Exercised at 40% RH	5.24±1.13^{^†}	4.73±1.26^{^†}	1.17±0.09^a,p	0.94±0.18	0.78±0.10	0.40±0.06^a	89.03±5.01	79.28±6.92	39.64±2.36^a,p
Exercised at 90% RH	2.49±0.22^{^*}^,^{^†}	3.62±0.59^{^†}	1.12±0.13^a,p	1.28±0.13	1.20±0.08^{^*}^,^{^†}	0.39±0.05^a,p	87.21±5.03	83.87±4.10	28.48±4.95^a,p,^{^*}^,^{^†}

	P_O₂ (kPa)					[O₂] (mmol l^-1)
	Pulmonary	Arterial	Venous	Pulmonary	Arterial	Venous	Pulmonary	Arterial	Venous
Resting	12.75±0.64	8.88±1.67^p	1.05±0.12^a,p	0.99±0.12	0.87±0.12	0.46±0.07^a	96.65±6.54	83.58±4.61	44.80±4.21^a,p
Exercised at 40% RH	5.24±1.13^{^†}	4.73±1.26^{^†}	1.17±0.09^a,p	0.94±0.18	0.78±0.10	0.40±0.06^a	89.03±5.01	79.28±6.92	39.64±2.36^a,p
Exercised at 90% RH	2.49±0.22^{^*}^,^{^†}	3.62±0.59^{^†}	1.12±0.13^a,p	1.28±0.13	1.20±0.08^{^*}^,^{^†}	0.39±0.05^a,p	87.21±5.03	83.87±4.10	28.48±4.95^a,p,^{^*}^,^{^†}

	P_CO₂ (kPa)					[CO₂] (mmol l^-1)
	Pulmonary	Arterial	Venous	Pulmonary	Arterial	Venous	Pulmonary	Arterial	Venous
Resting	1.70±0.09	1.37±0.17	1.56±0.10	16.47±1.89	17.49±1.90	17.34±1.81	7.63±0.04	7.60±0.04	7.59±0.04
Exercised at 40% RH	1.76±0.18	2.10±0.15^{^†}	2.24±0.16^{^†}	13.11±1.43	12.51±1.53^{^†}	13.86±1.15	7.49±0.02^{^†}	7.45±0.03^{^†}	7.35±0.04^{^†}
Exercised at 90% RH	2.14±0.22^{^†}	2.34±0.22^{^†}	2.69±0.19^{^*}^,^{^†}	12.20±0.81^{^†}	12.79±0.73^{^†}	11.89±0.62^{^†}	7.40±0.03^{^*}^,^{^†}	7.40±0.03^{^†}	7.35±0.03^{^†}

	P_CO₂ (kPa)					[CO₂] (mmol l^-1)
	Pulmonary	Arterial	Venous	Pulmonary	Arterial	Venous	Pulmonary	Arterial	Venous
Resting	1.70±0.09	1.37±0.17	1.56±0.10	16.47±1.89	17.49±1.90	17.34±1.81	7.63±0.04	7.60±0.04	7.59±0.04
Exercised at 40% RH	1.76±0.18	2.10±0.15^{^†}	2.24±0.16^{^†}	13.11±1.43	12.51±1.53^{^†}	13.86±1.15	7.49±0.02^{^†}	7.45±0.03^{^†}	7.35±0.04^{^†}
Exercised at 90% RH	2.14±0.22^{^†}	2.34±0.22^{^†}	2.69±0.19^{^*}^,^{^†}	12.20±0.81^{^†}	12.79±0.73^{^†}	11.89±0.62^{^†}	7.40±0.03^{^*}^,^{^†}	7.40±0.03^{^†}	7.35±0.03^{^†}

There were no significant differences between pulmonary and arterial samples. Significant differences compared with arterial samples are indicated by an `a', while differences compared with pulmonary are indicated by a `p'above the venous sample for each treatment group.

indicates a difference in values of crabs exercised at 90% compared with those at 40% RH

†

indicates a difference between crabs exercised at the respective humidity and those at rest. Differences between treatments were assessed by parametric analysis (ANOVA) with post-hoc testing (Tukey's HSD pair-wise comparison), while differences within treatments (i.e. haemolymph type) were assessed using Friedman's two-factor ANOVA for related samples, a non-parametric equivalent to one-factor ANOVA. N=8 for each treatment; total N=64

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