Table 1.

Primers used for RT-PCR of MHC-Eo (extraocular) and MHC-Neo(neonatal)

IsoformsForward primer (5′→3′)Reverse primer (5′→3′)Temperature (°C)PCR cycles
MHC-Eo TCGGGAGGAAGCAAGAAGGGCGGGA ATCACGCCAGGTGTCTTAGTCTCATTGGG 64 32 
MHC-Neo CGTAATGCTGAGGCTATTAAAGGCTT ACAAAGCAAGTGACCCAAAATAGCA 55 32 
IsoformsForward primer (5′→3′)Reverse primer (5′→3′)Temperature (°C)PCR cycles
MHC-Eo TCGGGAGGAAGCAAGAAGGGCGGGA ATCACGCCAGGTGTCTTAGTCTCATTGGG 64 32 
MHC-Neo CGTAATGCTGAGGCTATTAAAGGCTT ACAAAGCAAGTGACCCAAAATAGCA 55 32 
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