Taurine transport activity and the Na+ and Cl-dependence of muTAUT expressed in HEK-293T cells
| . | Taurine uptake (pmol/30 min/106 cells) . | . | |
|---|---|---|---|
| Medium . | Control vector . | pcDNA3.1 myc/His B-muTAUT . | |
| NaCl | 213±56.2 (100) | 811±32.1 (100) | |
| Na+ free (choline chloride) | 8.3±1.0 (3.9) | 10.0±2.0 (1.2) | |
| Cl- free (sodium gluconate) | 9.4±0.3 (4.4) | 17.0±1.3 (2.1) | |
| . | Taurine uptake (pmol/30 min/106 cells) . | . | |
|---|---|---|---|
| Medium . | Control vector . | pcDNA3.1 myc/His B-muTAUT . | |
| NaCl | 213±56.2 (100) | 811±32.1 (100) | |
| Na+ free (choline chloride) | 8.3±1.0 (3.9) | 10.0±2.0 (1.2) | |
| Cl- free (sodium gluconate) | 9.4±0.3 (4.4) | 17.0±1.3 (2.1) | |
Control vector (pcDNA3.1myc/His B) and cDNA construct (pcDNA3.1myc/His B-muTAUT) were introduced to HEK-293T cells, and uptake of 10 μmol 1-1 taurine containing 0.5 μCi ml-1[3H]taurine was measured. Values are mean ± standard deviation of four samples from a single experiment. The values in parentheses represent the percentage of the uptake in NaCl medium.