Table 1.

Relationship between glycolytic flux rate (J) and the activities of hexokinase (HK), cytochrome oxidase (COX) and glycogen phosphorylase (GP) in orchid bee flight muscle, analysed using conventional statistics and phylogenetically independent contrasts (PIC)

Conventional
PIC gradual
PIC speciational
EnzymeEquationr2Pbr2Pbr2P
HK 5.11J0.98 (0.72, 1.25) 0.83 <0.001 0.83 (0.34, 1.31) 0.59 <0.005 0.84 (0.25, 1.43) 0.50 <0.01 
COX 429.7J0.47 (0.24, 0.69) 0.61 <0.001  0.12 NS  0.19 NS 
GP 4.56J0.36 (0.07, 0.65) 0.38 <0.05  0.28 NS 0.66 (0.14, 1.18) 0.44 <0.05 
Conventional
PIC gradual
PIC speciational
EnzymeEquationr2Pbr2Pbr2P
HK 5.11J0.98 (0.72, 1.25) 0.83 <0.001 0.83 (0.34, 1.31) 0.59 <0.005 0.84 (0.25, 1.43) 0.50 <0.01 
COX 429.7J0.47 (0.24, 0.69) 0.61 <0.001  0.12 NS  0.19 NS 
GP 4.56J0.36 (0.07, 0.65) 0.38 <0.05  0.28 NS 0.66 (0.14, 1.18) 0.44 <0.05 

N=14 species for conventional analysis and 11 contrasts for PIC analysis.

For gradual evolution, branch lengths from fig. 1 in Darveau et al.(2005a) were used; for speciational model, branch lengths were set to 1.

NS, not significant.

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