Primers used for qRT-PCR
| Gene . | Direction . | Sequence (5′-3′) . |
|---|---|---|
| 14-3-3a | F | CAA CGA GGA GCG CAA CCT |
| R | CGG GCA CCC ACA ACA TTC | |
| CA2 | F | AGG GCT GAC GCT GAT TGG |
| R | CCT CTG CTG GAA AGC GTT ACC | |
| CFTR | F | AAT CGA GCA GTT CCC AGA CAA G |
| R | AGC TGT TTG TGC CCA TTG C | |
| EF1α | F | GGG AAA GGG CTC CTT CAA GT |
| R | ACG CTC GGC CTT CAG CTT | |
| Na+,K+- | F | ATT GCT GCC CGC CTG AA |
| ATPase α1a | R | TGA ACG ACG CAA GCT TTG G |
| NBC1 | F | AAA GGC GGA GAA CGT TGG A |
| R | CCA TCA AGC TGT GCA AAG ACA | |
| NHE2 | F | ACA GCA TCA GGC GCA TTC T |
| R | GCT GGC ATC TGC TTG TTG TTA A | |
| NHE3 | F | TGT TGA AGT CGT CAG CGA GAA |
| R | CAG CTC GTG GAA GAC GTT GA | |
| NKCC1 | F | CCC GCA GCC ACT GGT ATT |
| R | GCC ATC TGT GGG TCA GCA A | |
| V-ATPase | F | TGA AGT TCA AGG ACC CGG TTA |
| R | CTG CGC GTA CTC GCC TTT |
| Gene . | Direction . | Sequence (5′-3′) . |
|---|---|---|
| 14-3-3a | F | CAA CGA GGA GCG CAA CCT |
| R | CGG GCA CCC ACA ACA TTC | |
| CA2 | F | AGG GCT GAC GCT GAT TGG |
| R | CCT CTG CTG GAA AGC GTT ACC | |
| CFTR | F | AAT CGA GCA GTT CCC AGA CAA G |
| R | AGC TGT TTG TGC CCA TTG C | |
| EF1α | F | GGG AAA GGG CTC CTT CAA GT |
| R | ACG CTC GGC CTT CAG CTT | |
| Na+,K+- | F | ATT GCT GCC CGC CTG AA |
| ATPase α1a | R | TGA ACG ACG CAA GCT TTG G |
| NBC1 | F | AAA GGC GGA GAA CGT TGG A |
| R | CCA TCA AGC TGT GCA AAG ACA | |
| NHE2 | F | ACA GCA TCA GGC GCA TTC T |
| R | GCT GGC ATC TGC TTG TTG TTA A | |
| NHE3 | F | TGT TGA AGT CGT CAG CGA GAA |
| R | CAG CTC GTG GAA GAC GTT GA | |
| NKCC1 | F | CCC GCA GCC ACT GGT ATT |
| R | GCC ATC TGT GGG TCA GCA A | |
| V-ATPase | F | TGA AGT TCA AGG ACC CGG TTA |
| R | CTG CGC GTA CTC GCC TTT |
F, forward; R, reverse.