Primers used for cloning
| Gene . | Direction . | Sequence (5′-3′) . | Tm (°C) . | Species . | Accession no. . |
|---|---|---|---|---|---|
| CA2 | F | (C/T)CG (C/G)CA GTC (C/T)CC (C/T/A)(A/G)T (T/A/G)GA | 50 | Danio rerio | NM_199215.1 |
| R | CAG (A/T)GG (A/T/G/C)GG (T/A/G)GT GGT C(A/C)G (G/A)G | 50 | Mus musculus | NM_009801.3 | |
| Oncorhynchus mykiss | AY5414870 | ||||
| Platichthys flesus | AF093622 | ||||
| Tribolodon hakonensis | AB055617 | ||||
| NBC1 | F | GC(G/C) AG(G/A) TGG AT(A/T/C) AAG TTT GA(G/A) GA | 52 | Ambystoma tigrinum | AF001958 |
| R | GA(G/A/C) CG(C/A) AGG TTG GA(C/T) TT(C/T) TTG | 53 | Mus musculus | AF141934 | |
| Oncorhynchus mykiss | AF434166 | ||||
| Tribolodon hakonensis | AB055467 | ||||
| NHE2 | F | AA(C/T) GA(C/T) G(C/G)I GTI ACI GTI GT | See Towle et al. (1997) | ||
| R | GG IC(G/T) IAT IGT IAT ICC (C/T)TG | ||||
| NHE3 | F | GC(C/G/A/T) GT(G/T) CTG GC(C/G/A/T) GTI TT(C/T) GA(G/A) GA | See Hirata et al. (2003) | ||
| R | GC(C/G/A/T) CC(C/G/A/T) C(G/T)(C/G/A/T) A(G/A)I CCI CC(G/A) TA |
| Gene . | Direction . | Sequence (5′-3′) . | Tm (°C) . | Species . | Accession no. . |
|---|---|---|---|---|---|
| CA2 | F | (C/T)CG (C/G)CA GTC (C/T)CC (C/T/A)(A/G)T (T/A/G)GA | 50 | Danio rerio | NM_199215.1 |
| R | CAG (A/T)GG (A/T/G/C)GG (T/A/G)GT GGT C(A/C)G (G/A)G | 50 | Mus musculus | NM_009801.3 | |
| Oncorhynchus mykiss | AY5414870 | ||||
| Platichthys flesus | AF093622 | ||||
| Tribolodon hakonensis | AB055617 | ||||
| NBC1 | F | GC(G/C) AG(G/A) TGG AT(A/T/C) AAG TTT GA(G/A) GA | 52 | Ambystoma tigrinum | AF001958 |
| R | GA(G/A/C) CG(C/A) AGG TTG GA(C/T) TT(C/T) TTG | 53 | Mus musculus | AF141934 | |
| Oncorhynchus mykiss | AF434166 | ||||
| Tribolodon hakonensis | AB055467 | ||||
| NHE2 | F | AA(C/T) GA(C/T) G(C/G)I GTI ACI GTI GT | See Towle et al. (1997) | ||
| R | GG IC(G/T) IAT IGT IAT ICC (C/T)TG | ||||
| NHE3 | F | GC(C/G/A/T) GT(G/T) CTG GC(C/G/A/T) GTI TT(C/T) GA(G/A) GA | See Hirata et al. (2003) | ||
| R | GC(C/G/A/T) CC(C/G/A/T) C(G/T)(C/G/A/T) A(G/A)I CCI CC(G/A) TA |
F, forward; R, reverse; Tm, annealing temperature.