Skip to Main Content
Table 3.

Transmembrane transport of Chlorophenol Red into the lumen of the gut and Malpighian tubules of larvae after freezing at−20°C for 40 days

Coloration in the lumen
Time of incubation (min)FG
MGP
MTA (cell)
MTP
SDSDSDSD
− − − − − − − − 
10 ± − − 
20 ++ ++ ++ ++ ± ++ ± 
30 ++ +++ ++ ++ ++ 
60 ++ +++ ++ ++ ++ 
Coloration in the lumen
Time of incubation (min)FG
MGP
MTA (cell)
MTP
SDSDSDSD
− − − − − − − − 
10 ± − − 
20 ++ ++ ++ ++ ± ++ ± 
30 ++ +++ ++ ++ ++ 
60 ++ +++ ++ ++ ++ 

Larvae were collected on Nov. 13, 2001 and frozen for 40 days at−20°C. Larvae were then thawed at 22°C for 5h before tissues were removed and incubated in Chlorophenol Red-Coast's solution. S, survived;D, died.

Tissues and coloration scores were the same as Table 1 (N=9).

Close Modal

or Create an Account

Close Modal
Close Modal