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Table 3.

Transmembrane transport of Chlorophenol Red into the lumen of the gut and Malpighian tubules of larvae after freezing at−20°C for 40 days

	Coloration in the lumen
Time of incubation (min)	FG			MGP			MTA (cell)			MTP
Time of incubation (min)		S	D	S	D	S	D	S	D
5	−	−	−	−	−	−	−	−
10	+	±	+	+	+	−	+	−
20	++	+	++	++	++	±	++	±
30	++	+	+++	++	++	+	++	+
60	++	+	+++	++	++	+	++	+

	Coloration in the lumen
Time of incubation (min)	FG			MGP			MTA (cell)			MTP
Time of incubation (min)		S	D	S	D	S	D	S	D
5	−	−	−	−	−	−	−	−
10	+	±	+	+	+	−	+	−
20	++	+	++	++	++	±	++	±
30	++	+	+++	++	++	+	++	+
60	++	+	+++	++	++	+	++	+

Larvae were collected on Nov. 13, 2001 and frozen for 40 days at−20°C. Larvae were then thawed at 22°C for 5h before tissues were removed and incubated in Chlorophenol Red-Coast's solution. S, survived;D, died.

Tissues and coloration scores were the same as Table 1 (N=9).

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