The Company of Biologists

Table 2.

Effect of temperature on transmembrane transport of Chlorophenol Red into the lumen of the gut and Malpighian tubules

		Transport (coloration)
		Gut			Malpighian tubules
Temperature (°C)	Incubation (min)	FG	MGP	MTA (cell)	MTP
22	5	+	+	+	+
	10	+++	+++	+++	+++
10	5	−	−	−	−
	10	+	+	±	±
	20	+	++	+	+
	30	++	+++	+++	+++
0	10	−	−	−	−
	20	+	+	−	−
	30	+	++	±	±
	40	++	++	++	++
	60	++	+++	+++	+++

		Transport (coloration)
		Gut			Malpighian tubules
Temperature (°C)	Incubation (min)	FG	MGP	MTA (cell)	MTP
22	5	+	+	+	+
	10	+++	+++	+++	+++
10	5	−	−	−	−
	10	+	+	±	±
	20	+	++	+	+
	30	++	+++	+++	+++
0	10	−	−	−	−
	20	+	+	−	−
	30	+	++	±	±
	40	++	++	++	++
	60	++	+++	+++	+++

Tissues and coloration scores were the same as Table 1 (N=9).