. | Transport (coloration) . | . | . | . | . | . | . | . | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Gut . | . | . | . | Malpighian tubules . | . | . | . | |||||||
. | FG . | MGA . | MGP . | HG . | MTU . | MTA (cell) . | MTA (cry) . | MTP . | |||||||
Control | +++ | − | +++ | − | +++ | +++ | − | +++ | |||||||
+KCN | ++ | − | ++ | − | + | ± | − | ± | |||||||
+Ouabain | ++ | − | ++ | − | ++ | + | − | + |
. | Transport (coloration) . | . | . | . | . | . | . | . | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Gut . | . | . | . | Malpighian tubules . | . | . | . | |||||||
. | FG . | MGA . | MGP . | HG . | MTU . | MTA (cell) . | MTA (cry) . | MTP . | |||||||
Control | +++ | − | +++ | − | +++ | +++ | − | +++ | |||||||
+KCN | ++ | − | ++ | − | + | ± | − | ± | |||||||
+Ouabain | ++ | − | ++ | − | ++ | + | − | + |
Observations were made for 20 minutes at 22°C (N=9)
All experiments were conducted in Coast's solution; the concentration of both KCN and ouabain was 0.01 mol 1−1. FG, foregut; MGA,midgut anterior portion; MGP, midgut posterior portion; HG, hindgut; MTU,Malpighian tubules ureter; MTA (cell), proximal region of anterior Malpighian tubules (cell); MTA (cry), distal region of anterior Malpighian tubules(crystal); MTP, posterior Malpighian tubules.
Red color increased in the lumen with time. Coloration was scored as:−, no color; ±, very slight color; +, positive color; ++, more color; +++, very strong color.