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Table 1.

Effect of inhibitors on transmembrane transport of Chlorophenol Red into the lumen of the gut and Malpigian tubules using larvae collected on Nov. 13, 2001

Transport (coloration)
Gut
Malpighian tubules
FGMGAMGPHGMTUMTA (cell)MTA (cry)MTP
Control +++ − +++ − +++ +++ − +++ 
+KCN ++ − ++ − ± − ± 
+Ouabain ++ − ++ − ++ − 
Transport (coloration)
Gut
Malpighian tubules
FGMGAMGPHGMTUMTA (cell)MTA (cry)MTP
Control +++ − +++ − +++ +++ − +++ 
+KCN ++ − ++ − ± − ± 
+Ouabain ++ − ++ − ++ − 

Observations were made for 20 minutes at 22°C (N=9)

All experiments were conducted in Coast's solution; the concentration of both KCN and ouabain was 0.01 mol 1−1. FG, foregut; MGA,midgut anterior portion; MGP, midgut posterior portion; HG, hindgut; MTU,Malpighian tubules ureter; MTA (cell), proximal region of anterior Malpighian tubules (cell); MTA (cry), distal region of anterior Malpighian tubules(crystal); MTP, posterior Malpighian tubules.

Red color increased in the lumen with time. Coloration was scored as:−, no color; ±, very slight color; +, positive color; ++, more color; +++, very strong color.

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