Forward and reverse primers used for quantifying calpains, ecdysone receptor and elongation factor 2 mRNAs by realtime PCR
| Name . | Sequence . | Tm (°C) . | Location in cDNA (bp #) . |
|---|---|---|---|
| RcEF2 F1 | 5′-TTCTATGCCTTTGGCCGTGTCTTCTC-3′ | 62.6 | 11-36 |
| RcEF2 R1 | 5′-TGATGGTGCCCGTCTTAACCAGATAC-3′ | 62.1 | 214-239 |
| RcCalpM F1 | 5′-GGCGGCTGCAGGAATTACATTAACAC-3′ | 62.2 | 1498-1523 |
| RcCalpM R1 | 5′-TGTACCTGAAGAAATCGACGTCCAGC-3′ | 62.2 | 1713-1738 |
| RcCalpB F1 | 5′-GTTCAACTTTGAGGGCTTCAGCAAGG-3′ | 62.2 | 2023-2048 |
| RcCalpB R1 | 5′-GATAACCAGCAGAGTTGAGGGCTTGA-3′ | 62.3 | 2209-2234 |
| RcCalpT F1 | 5′-TCTCTGATGTGCTGTGCCATAACTCC-3′ | 62.3 | 973-998 |
| RcCalpT R1 | 5′-TGATGCAGAGACCTGTGACCATTCTG-3′ | 62.2 | 1202-1227 |
| RcEcR F1 | 5′-CACGAAGAATGCCGTGTACCAGTGTA-3′ | 62.3 | 35-60 |
| RcEcR R1 | 5′-CATCTGCTTCAGTTGGCTGCTCAAAC-3′ | 62.4 | 380-405 |
| Name . | Sequence . | Tm (°C) . | Location in cDNA (bp #) . |
|---|---|---|---|
| RcEF2 F1 | 5′-TTCTATGCCTTTGGCCGTGTCTTCTC-3′ | 62.6 | 11-36 |
| RcEF2 R1 | 5′-TGATGGTGCCCGTCTTAACCAGATAC-3′ | 62.1 | 214-239 |
| RcCalpM F1 | 5′-GGCGGCTGCAGGAATTACATTAACAC-3′ | 62.2 | 1498-1523 |
| RcCalpM R1 | 5′-TGTACCTGAAGAAATCGACGTCCAGC-3′ | 62.2 | 1713-1738 |
| RcCalpB F1 | 5′-GTTCAACTTTGAGGGCTTCAGCAAGG-3′ | 62.2 | 2023-2048 |
| RcCalpB R1 | 5′-GATAACCAGCAGAGTTGAGGGCTTGA-3′ | 62.3 | 2209-2234 |
| RcCalpT F1 | 5′-TCTCTGATGTGCTGTGCCATAACTCC-3′ | 62.3 | 973-998 |
| RcCalpT R1 | 5′-TGATGCAGAGACCTGTGACCATTCTG-3′ | 62.2 | 1202-1227 |
| RcEcR F1 | 5′-CACGAAGAATGCCGTGTACCAGTGTA-3′ | 62.3 | 35-60 |
| RcEcR R1 | 5′-CATCTGCTTCAGTTGGCTGCTCAAAC-3′ | 62.4 | 380-405 |
Abbreviations: EcR, ecdysone receptor; EF2, elongation factor 2; Tm, melting temperature.
GenBank accession numbers: Gl-EF2, AY552550; Gl-CalpM, AY639152; Gl-CalpB,AY639153; Gl-CalpT, AY639154; Gl-EcR, AY642975.