Primers used in 3′ and 5′ RACE for cloning land crab calpains B, M and T and ecdysone receptor
| Name . | Sequence . | Tm (°C) . | Description . | Location in cDNA (bp #) . |
|---|---|---|---|---|
| cCalpM R1 | 5′-CCGTTGGACATGATGCTTTCTGG-3′ | 59.9 | Gl-CalpM 5′ RACE first round RP | 1081-1103 |
| cCalpM R2 | 5′-TCCACCATACTCTCGTTGATGTTC-3′ | 58.0 | Gl-CalpM 5′ RACE nested RP | 924-947 |
| cCalpM F1 | 5′-GGCGGGAACATCAACGAGAG-3′ | 59.0 | Gl-CalpM 3′ RACE first round FP | 918-937 |
| cCalpM F2 | 5′-CTCGCTCATAGGTTGTGCCATCG-3′ | 61.2 | Gl-CalpM 3′ RACE nested FP | 1034-1056 |
| cCalpB R1 | 5′-CAGTAAAGTCCTCCATTGCCTCACAG-3′ | 60.9 | Gl-CalpB 5′ RACE first round RP | 865-920 |
| cCalpB R2 | 5′-TTGGTCACTAGGCACTATTTGATAGAAG-3′ | 58.2 | Gl-CalpB 5′ RACE nested RP | 655-682 |
| cCalpB F1 | 5′-GCTATGATGGATGTGGATCGCTC-3′ | 58.8 | Gl-CalpB 3′ RACE first round FP | 2069-2091 |
| cCalpB F2 | 5′-GATGACTTCATCATGTGCTCTGTG-3′ | 57.4 | Gl-CalpB 3′ RACE nested FP | 2303-2326 |
| cCalpT R1 | 5′-GAAGGTGAGGATGGTGCGTCTCAAGA-3′ | 63.9 | Gl-CalpT 5′ RACE first round RP | 1343-1365 |
| cCalpT R2 | 5′-TATGTTGATTTTGCGGCAAGCGGTGATTC-3′ | 64.0 | Gl-CalpT 5′ RACE nested RP | 1060-1088 |
| cCalpT F1 | 5′-GACGATTGGAGGCTATGTGGATGAC-3′ | 60.7 | Gl-CalpT 3′ RACE first round FP | 894-918 |
| cCalpT F2 | 5′-CTCTCTGATGTGCTGTGCCATAACTCC-3′ | 62.6 | Gl-CalpT 3′ RACE nested FP | 972-998 |
| cEcR F1 | 5′-GTTTGAGCAGCCAACTGAAGCAGATG-3′ | 62.3 | EcR 3′ RACE first round FP | 380-405 |
| cEcR F2 | 5′-ATTCAGGCACATAACCGAGATGACGATC-3′ | 62.2 | EcR 3′ RACE nested FP | 455-482 |
| cEcR R1 | 5′-CATCTGCTTCAGTTGGCTGCTCAAAC-3′ | 62.3 | EcR 5′ RACE first round RP | 380-405 |
| cEcR R2 | 5′-CACTGGACTAATAGGAGCAGCCTTGTC-3′ | 62.2 | EcR 5′ RACE nested RP | 258-284 |
| Name . | Sequence . | Tm (°C) . | Description . | Location in cDNA (bp #) . |
|---|---|---|---|---|
| cCalpM R1 | 5′-CCGTTGGACATGATGCTTTCTGG-3′ | 59.9 | Gl-CalpM 5′ RACE first round RP | 1081-1103 |
| cCalpM R2 | 5′-TCCACCATACTCTCGTTGATGTTC-3′ | 58.0 | Gl-CalpM 5′ RACE nested RP | 924-947 |
| cCalpM F1 | 5′-GGCGGGAACATCAACGAGAG-3′ | 59.0 | Gl-CalpM 3′ RACE first round FP | 918-937 |
| cCalpM F2 | 5′-CTCGCTCATAGGTTGTGCCATCG-3′ | 61.2 | Gl-CalpM 3′ RACE nested FP | 1034-1056 |
| cCalpB R1 | 5′-CAGTAAAGTCCTCCATTGCCTCACAG-3′ | 60.9 | Gl-CalpB 5′ RACE first round RP | 865-920 |
| cCalpB R2 | 5′-TTGGTCACTAGGCACTATTTGATAGAAG-3′ | 58.2 | Gl-CalpB 5′ RACE nested RP | 655-682 |
| cCalpB F1 | 5′-GCTATGATGGATGTGGATCGCTC-3′ | 58.8 | Gl-CalpB 3′ RACE first round FP | 2069-2091 |
| cCalpB F2 | 5′-GATGACTTCATCATGTGCTCTGTG-3′ | 57.4 | Gl-CalpB 3′ RACE nested FP | 2303-2326 |
| cCalpT R1 | 5′-GAAGGTGAGGATGGTGCGTCTCAAGA-3′ | 63.9 | Gl-CalpT 5′ RACE first round RP | 1343-1365 |
| cCalpT R2 | 5′-TATGTTGATTTTGCGGCAAGCGGTGATTC-3′ | 64.0 | Gl-CalpT 5′ RACE nested RP | 1060-1088 |
| cCalpT F1 | 5′-GACGATTGGAGGCTATGTGGATGAC-3′ | 60.7 | Gl-CalpT 3′ RACE first round FP | 894-918 |
| cCalpT F2 | 5′-CTCTCTGATGTGCTGTGCCATAACTCC-3′ | 62.6 | Gl-CalpT 3′ RACE nested FP | 972-998 |
| cEcR F1 | 5′-GTTTGAGCAGCCAACTGAAGCAGATG-3′ | 62.3 | EcR 3′ RACE first round FP | 380-405 |
| cEcR F2 | 5′-ATTCAGGCACATAACCGAGATGACGATC-3′ | 62.2 | EcR 3′ RACE nested FP | 455-482 |
| cEcR R1 | 5′-CATCTGCTTCAGTTGGCTGCTCAAAC-3′ | 62.3 | EcR 5′ RACE first round RP | 380-405 |
| cEcR R2 | 5′-CACTGGACTAATAGGAGCAGCCTTGTC-3′ | 62.2 | EcR 5′ RACE nested RP | 258-284 |
Abbreviations: EcR, ecdysone receptor; FP, forward primer; RP, reverse primer; Tm, melting temperature.