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Table 8.

Fatty acid composition of the six phospholipid classes examined in the skin of P. schlosseri exposed for 6 days to 50%SW containing 10 mmol l-1 Tris buffer, pH 7.2, with or without (control) 30 mmol l-1 NH4Cl

Fatty acid composition (% of total in the class)
Phospholipid classControlAmmonia exposed
SM   
   Tot sat 78.4±0.5 79.7±1.0 
   Tot mono 5.64±0.87 3.48±0.90 
   Tot poly 16.0±0.5 16.9±0.6 
   n3 
   n6 16.0±0.5 16.9±0.6 
PC   
   Tot sat 44.0±0.7 42.3±0.6 
   Tot mono 44.7±0.3 44.6±0.4 
   Tot poly 11.3±0.5 13.1±0.5* 
   n3 1.90±0.32 2.26±0.03 
   n6 9.42±0.47 10.8±0.5* 
PS   
   Tot sat 71.3±2.1 70.6±0.7 
   Tot mono 12.8±0.6 12.2±0.3 
   Tot poly 15.8±2.0 17.2±0.8 
   n3 4.44±0.96 3.86±0.19 
   n6 11.4±1.2 13.3±0.8 
PI   
   Tot sat 60.6±1.0 63.7±0.7 
   Tot mono 9.07±1.09 9.91±0.91 
   Tot poly 30.4±0.8 26.4±0.9* 
   n3 
   n6 30.4±0.8 26.4±0.9* 
PE   
   Tot sat 44.7±2.6 42.2±2.0 
   Tot mono 18.7±1.1 18.9±1.3 
   Tot poly 36.6±3.5 38.9±3.1 
   n3 6.7±1.7 10.2±1.4 
   n6 29.9±3.1 28.6±3.2 
CL   
   Tot sat 41.5±5.0 30.8±6.7 
   Tot mono 32.5±3.2 30.5±5.8 
   Tot poly 26.0±6.7 38.8±3.5 
   n3 0.60±0.60 
   n6 26.0±6.7 38.1±3.7 
Fatty acid composition (% of total in the class)
Phospholipid classControlAmmonia exposed
SM   
   Tot sat 78.4±0.5 79.7±1.0 
   Tot mono 5.64±0.87 3.48±0.90 
   Tot poly 16.0±0.5 16.9±0.6 
   n3 
   n6 16.0±0.5 16.9±0.6 
PC   
   Tot sat 44.0±0.7 42.3±0.6 
   Tot mono 44.7±0.3 44.6±0.4 
   Tot poly 11.3±0.5 13.1±0.5* 
   n3 1.90±0.32 2.26±0.03 
   n6 9.42±0.47 10.8±0.5* 
PS   
   Tot sat 71.3±2.1 70.6±0.7 
   Tot mono 12.8±0.6 12.2±0.3 
   Tot poly 15.8±2.0 17.2±0.8 
   n3 4.44±0.96 3.86±0.19 
   n6 11.4±1.2 13.3±0.8 
PI   
   Tot sat 60.6±1.0 63.7±0.7 
   Tot mono 9.07±1.09 9.91±0.91 
   Tot poly 30.4±0.8 26.4±0.9* 
   n3 
   n6 30.4±0.8 26.4±0.9* 
PE   
   Tot sat 44.7±2.6 42.2±2.0 
   Tot mono 18.7±1.1 18.9±1.3 
   Tot poly 36.6±3.5 38.9±3.1 
   n3 6.7±1.7 10.2±1.4 
   n6 29.9±3.1 28.6±3.2 
CL   
   Tot sat 41.5±5.0 30.8±6.7 
   Tot mono 32.5±3.2 30.5±5.8 
   Tot poly 26.0±6.7 38.8±3.5 
   n3 0.60±0.60 
   n6 26.0±6.7 38.1±3.7 

The composition of each phopholipid class is expressed as % of the total content in that class.

PC, phosphatidylcholine; PS, phosphatidylserine; PI, phosphatidylinositol;PE, phosphatidylethanolamine; CL, cardiolipin; SM, sphingomyelin.

Tot sat, total saturated fatty acids; Tot mono, total monounsaturated fatty acids; Tot poly, or PUFAs, total polyunsaturated fatty acids; n3, n3 PUFAs;n6, PUFAs.

Values are means ± s.e.m. (N=5).

*

Significantly different from the corresponding control value

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