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Table 2.

The presence of neurotransmitters in the gut of zebrafish at different d.p.f. stages and in adult specimens

		Intestinal area
Antiserum	d.p.f.	Proximal	Middle	Distal
PACAP-38	2	+	+	-
	3	+	+	+
	5	+	+	+
	7	+	+	+, o
	Adult	+++, o	+++, o	+++, o
NKA 2809	2	+	-	-
	3	++	++	-
	5	++	++	++, o
	7	++	++	++, oo
	Adult	++, o	++, o	++, o

		Intestinal area
Antiserum	d.p.f.	Proximal	Middle	Distal
PACAP-38	2	+	+	-
	3	+	+	+
	5	+	+	+
	7	+	+	+, o
	Adult	+++, o	+++, o	+++, o
NKA 2809	2	+	-	-
	3	++	++	-
	5	++	++	++, o
	7	++	++	++, oo
	Adult	++, o	++, o	++, o

Proximal (intestinal bulb), middle and distal parts of the gastrointestinal tract of embryo, larvae and adult zebrafish were investigated using immunohistochemistry. The occurrence of nerves (+) and endocrine cells (o) was assessed on a three-step scale (-, none; +, occasional; ++, moderate; +++,abundant).

d.p.f., days post fertilization; PACAP, pituitary adenylate cyclase-activating polypeptide; NKA, neurokinin A.

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