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Table 2.

The presence of neurotransmitters in the gut of zebrafish at different d.p.f. stages and in adult specimens

Intestinal area
Antiserumd.p.f.ProximalMiddleDistal
PACAP-38 
 
 
 +, o 
 Adult +++, o +++, o +++, o 
NKA 2809 
 ++ ++ 
 ++ ++ ++, o 
 ++ ++ ++, oo 
 Adult ++, o ++, o ++, o 
Intestinal area
Antiserumd.p.f.ProximalMiddleDistal
PACAP-38 
 
 
 +, o 
 Adult +++, o +++, o +++, o 
NKA 2809 
 ++ ++ 
 ++ ++ ++, o 
 ++ ++ ++, oo 
 Adult ++, o ++, o ++, o 

Proximal (intestinal bulb), middle and distal parts of the gastrointestinal tract of embryo, larvae and adult zebrafish were investigated using immunohistochemistry. The occurrence of nerves (+) and endocrine cells (o) was assessed on a three-step scale (-, none; +, occasional; ++, moderate; +++,abundant).

d.p.f., days post fertilization; PACAP, pituitary adenylate cyclase-activating polypeptide; NKA, neurokinin A.

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