. | Activity (μmol min-1 ml-1 digestive juice) . | . | . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|
Enzyme . | pH 4 . | pH 5.5 . | pH 7 . | pH 8 . | pH 9 . | ||||
Total cellulase | |||||||||
G. natalis | 0.018±0.016a | 0.043±0.016b | 0.017±0.010a | 0.021±0.020a | 0.011±0.011a | ||||
D. hirtipes | 0.014±0.005a | 0.020±0.005a | 0.023±0.006a | 0.005±0.002a | 0.010±0.003a | ||||
β-1,4-glucosidase | |||||||||
G. natalis | 0.397±0.253a,b | 0.521±0.253a | 0.254±0.131b,c | 0.075±0.032c,d | 0.031±0.019d | ||||
D. hirtipes | 1.779±0.083a | 1.729±0.066a | 1.961±0.074a | 0.214±0.046b | 0.121±0.028b | ||||
Endo-β-1,4-glucanase | |||||||||
G. natalis | 0.297±0.174a | 2.076±0.104b | 2.304±0.116b | 0.945±0.189c | 1.981±0.106b | ||||
D. hirtipes | 0.627±0.092a | 1.534±0.206b | 1.434±0.101b | 0.710±0.169a | 1.041±0.151a,b |
. | Activity (μmol min-1 ml-1 digestive juice) . | . | . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|
Enzyme . | pH 4 . | pH 5.5 . | pH 7 . | pH 8 . | pH 9 . | ||||
Total cellulase | |||||||||
G. natalis | 0.018±0.016a | 0.043±0.016b | 0.017±0.010a | 0.021±0.020a | 0.011±0.011a | ||||
D. hirtipes | 0.014±0.005a | 0.020±0.005a | 0.023±0.006a | 0.005±0.002a | 0.010±0.003a | ||||
β-1,4-glucosidase | |||||||||
G. natalis | 0.397±0.253a,b | 0.521±0.253a | 0.254±0.131b,c | 0.075±0.032c,d | 0.031±0.019d | ||||
D. hirtipes | 1.779±0.083a | 1.729±0.066a | 1.961±0.074a | 0.214±0.046b | 0.121±0.028b | ||||
Endo-β-1,4-glucanase | |||||||||
G. natalis | 0.297±0.174a | 2.076±0.104b | 2.304±0.116b | 0.945±0.189c | 1.981±0.106b | ||||
D. hirtipes | 0.627±0.092a | 1.534±0.206b | 1.434±0.101b | 0.710±0.169a | 1.041±0.151a,b |
Total cellulase and β-1,4-glucosidase activities were measured as the rate of glucose production from the hydrolysis of either crystalline cellulose or cellobiose, respectively. Endo-β-1,4-glucanase activities were measured as the rate of reducing sugars produced from the hydrolysis of carboxymethyl cellulose. Within a row, similar superscript letters indicate similar means (P>0.05). Values are means ± s.e.m. (N=10).