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Table 1.

`Day' effects and repeatability of wheel-running at 25°C

	Day effect					Repeatability
	2 days F_2,37,P	3 days F_2,12,P	4 days F_2,3,P	Days 1-2 r,F_1,30,P	Days 1-3 r,F_1,13,P	Days 1-4 r,F_1,4,P
DMR	0.215,0.645	0.494,0.616	0.201,0.894	0.54,16.2,0.00013	0.13,0.231,0.319	0.39,0.718,0.222
RMR₅	2.59,0.116	0.093,0.911	0.460,0.714	0.62,23.8,0.00001	0.55,6.19,0.0131	0.71,5.06,0.0372
RMR₃₀	0.585,0.449	0.267,0.768	0.151,0.928	0.55,17.0,0.00009	0.56,6.24,0.0127	0.76,7.00,0.0228
V̇_O₂1	4.75,0.0355	1.21,0.314	0.183,0.906	0.64,27.5,<0.00001	0.77,20.3,0.00025	0.74,6.20,0.0265
V̇_O₂2	5.15,0.0290	1.98,0.159	0.447,0.723	0.69,35.0,<0.00001	0.76,19.1,0.00033	0.77,7.43,0.0208
V̇_O₂5	6.64,0.0140	1.58,0.226	0.449,0.721	0.71,38.4,<0.00001	0.83,30.7,0.00004	0.50,1.67,0.127
V̇_O₂10	4.48,0.0410	2.15,0.137	0.268,0.847	0.75,60.7,<0.00001	0.68,12.0,0.00198	0.50,1.64,0.126
D_run	0.0004,0.988	1.08,0.345	0.511,0.583	0.39,7.13,0.0055	0.77,20.7,0.00023	0.86,13.6,0.0070
T	2.63,0.113	0.740,0.486	0.714,0.559	0.48,11.9,0.00068	0.77,20.7,0.00023	0.84,12.3,0.0086
V_mean	18.1,0.00013	5.48,0.00984	1.33,0.301	0.42,8.10,0.00351	0.74,16.5,0.00058	0.30,2.11,0.103
V_max	2.40,0.129	1.44,0.255	0.435,0.731	0.79,63.9,<0.00001	0.86,38.2,0.00001	0.73,5.83,0.0303
V1	6.09,0.0181	2.44,0.105	0.623,0.611	0.67,31.9,<0.00001	0.80,24.9,0.00010	0.40,0.963,0.186
V2	6.57,0.0144	2.90,0.072	0.762,0.533	0.61,22.6,0.00002	0.74,17.0,0.00052	0.46,1.35,0.149
V5	5.61,0.0229	2.25,0.124	0.994,0.422	0.55,17.3,0.00008	0.76,18.6,0.00036	0.58,2.59,0.089
V10	3.83,0.058	1.56,0.227	0.987,0.425	0.57,18.6,0.00006	0.79,22.5,0.00016	0.71,5.07,0.0371
Bouts	25.8,<0.00001	1.09,0.336	5.17,0.0118	0.61,23.6,0.00001	0.48,4.10,0.031	0.916,26.2,0.00181
D_bout	9.36,0.0120	1.04,0.347	0.621,0.612	0.18,0.546,0.232	0.47,3.92,0.034	0.11,0.057,0.411

	Day effect					Repeatability
	2 days F_2,37,P	3 days F_2,12,P	4 days F_2,3,P	Days 1-2 r,F_1,30,P	Days 1-3 r,F_1,13,P	Days 1-4 r,F_1,4,P
DMR	0.215,0.645	0.494,0.616	0.201,0.894	0.54,16.2,0.00013	0.13,0.231,0.319	0.39,0.718,0.222
RMR₅	2.59,0.116	0.093,0.911	0.460,0.714	0.62,23.8,0.00001	0.55,6.19,0.0131	0.71,5.06,0.0372
RMR₃₀	0.585,0.449	0.267,0.768	0.151,0.928	0.55,17.0,0.00009	0.56,6.24,0.0127	0.76,7.00,0.0228
V̇_O₂1	4.75,0.0355	1.21,0.314	0.183,0.906	0.64,27.5,<0.00001	0.77,20.3,0.00025	0.74,6.20,0.0265
V̇_O₂2	5.15,0.0290	1.98,0.159	0.447,0.723	0.69,35.0,<0.00001	0.76,19.1,0.00033	0.77,7.43,0.0208
V̇_O₂5	6.64,0.0140	1.58,0.226	0.449,0.721	0.71,38.4,<0.00001	0.83,30.7,0.00004	0.50,1.67,0.127
V̇_O₂10	4.48,0.0410	2.15,0.137	0.268,0.847	0.75,60.7,<0.00001	0.68,12.0,0.00198	0.50,1.64,0.126
D_run	0.0004,0.988	1.08,0.345	0.511,0.583	0.39,7.13,0.0055	0.77,20.7,0.00023	0.86,13.6,0.0070
T	2.63,0.113	0.740,0.486	0.714,0.559	0.48,11.9,0.00068	0.77,20.7,0.00023	0.84,12.3,0.0086
V_mean	18.1,0.00013	5.48,0.00984	1.33,0.301	0.42,8.10,0.00351	0.74,16.5,0.00058	0.30,2.11,0.103
V_max	2.40,0.129	1.44,0.255	0.435,0.731	0.79,63.9,<0.00001	0.86,38.2,0.00001	0.73,5.83,0.0303
V1	6.09,0.0181	2.44,0.105	0.623,0.611	0.67,31.9,<0.00001	0.80,24.9,0.00010	0.40,0.963,0.186
V2	6.57,0.0144	2.90,0.072	0.762,0.533	0.61,22.6,0.00002	0.74,17.0,0.00052	0.46,1.35,0.149
V5	5.61,0.0229	2.25,0.124	0.994,0.422	0.55,17.3,0.00008	0.76,18.6,0.00036	0.58,2.59,0.089
V10	3.83,0.058	1.56,0.227	0.987,0.425	0.57,18.6,0.00006	0.79,22.5,0.00016	0.71,5.07,0.0371
Bouts	25.8,<0.00001	1.09,0.336	5.17,0.0118	0.61,23.6,0.00001	0.48,4.10,0.031	0.916,26.2,0.00181
D_bout	9.36,0.0120	1.04,0.347	0.621,0.612	0.18,0.546,0.232	0.47,3.92,0.034	0.11,0.057,0.411

Metabolic variables(V̇_O₂, DMR, RMR)were analyzed with repeated-measures ANCOVA (sex as a fixed factor, body mass as covariate) and behavioral variables with repeated-measures ANOVA (sex as a fixed factor; body mass had no effect). Repeatability was assessed from residuals (Pearson's r between pairs of days).

N=41 for days 1 and 2, 16 for day 3, and 7 for day 4.

DMR, daily energy expenditure; RMR₅, RMR₃₀, lowest V̇_O₂ averaged over 5 or 30 min; D_run, distance covered in 24 h; T,time spent running in 24 h; V_mean, average speed; V_max, highest speed; V1, V2, V5, V10, highest speed averaged over 1, 2, 5 and 10 min,respectively; Bouts, number of running bouts (see text); D_bout, mean bout duration; V̇_O₂1, V̇_O₂2, V̇_O₂5, V̇_O₂10, highest V̇_O₂ averaged over 1, 2, 5, and 10 min, respectively.

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